State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, PR China; School of Environment, Harbin Institute of Technology, Harbin 150090, PR China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, PR China; School of Environment, Harbin Institute of Technology, Harbin 150090, PR China.
Bioresour Technol. 2021 Jun;330:124972. doi: 10.1016/j.biortech.2021.124972. Epub 2021 Mar 11.
To understand the microbial social behaviors regulated by acyl-homoserine lactones (AHLs) in the upflow anaerobic sludge blanket (UASB) during the restored process after a shock loading, the correlation analyses of AHLs and components of extracellular polymeric substances (EPS), AHLs genes and microbes, and AHLs and microbes were investigated. The results showed that the performance could be restored by regulating influent organic loading rate stage-by-stage. A variation in microbial community and endogenous AHLs was also found during the restoration process. It was found that C-HSL had improved the synthesis of protein in EPS and resulted in better aggregation of microbes. C-HSL, as well as C-HSL and 3-oxoC-HSL, could prompt the metabolism of acidogenic fermentation bacteria. While 3-oxoC-HSL was identified as the key signal molecule in enhancing methanogenesis. The present work advanced the understanding of microbial social behaviors and provided an attractive strategy for the restoration of anaerobic digestion after shock loadings.
为了理解在上流式厌氧污泥床（UASB）在冲击负荷后恢复过程中酰基高丝氨酸内酯（AHLs）调节的微生物社会行为，研究了 AHLs 与胞外聚合物（EPS）成分、AHLs 基因和微生物以及 AHLs 和微生物之间的相关性。结果表明，可以通过逐步调节进水有机负荷率来恢复性能。在恢复过程中还发现了微生物群落和内源性 AHLs 的变化。发现 C-HSL 可以促进 EPS 中蛋白质的合成，从而使微生物更好地聚集。C-HSL 以及 C-HSL 和 3-氧代 C-HSL 可以促进产酸发酵菌的代谢。而 3-氧代 C-HSL 被鉴定为增强产甲烷作用的关键信号分子。本研究增进了对微生物社会行为的理解，并为冲击负荷后厌氧消化的恢复提供了有吸引力的策略。
