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回流比对膨胀颗粒污泥床反应器中厌氧污泥和微生物社会行为的影响:从酰高丝氨酸内酯介导的群体感应角度。

Effects of reflux ratio on the anaerobic sludge and microbial social behaviors in an expanded granular sludge bed reactor: From the perspective of acyl-homoserine lactones-mediated quorum sensing.

机构信息

State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, PR China.

State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, PR China.

出版信息

Bioresour Technol. 2021 Oct;337:125360. doi: 10.1016/j.biortech.2021.125360. Epub 2021 Jun 2.

Abstract

Performance of anaerobic sludge and microbial social behaviors in an expanded granular sludge bed (EGSB) were evaluated by increasing reflux ratio from 50% to 500% stage by stage, with a constant influent chemical oxygen demand (COD) of 5500 mg/L at hydraulic retention time 12 h. The results indicated that the reflux ratio of 100% - 200% was more favorable for the EGSB with a methane production of 2.4 m/m·d. It was found that acyl-homoserine lactones (AHLs)-mediated quorum sensing (QS) could balance various microbial populations in the anaerobic digestion process. C-HSL and C-HSL were identified as the specific AHLs in enhancing granulation of anaerobic sludge by stimulating protein secretion into extracellular polymeric substances (EPS). 3-oxo-C-HSL and 3-oxo-C-HSL were verified for the enhancement of methanogenesis. The present study showed a novel perspective on the performance of EGSB with reflux ratios based on the AHLs-mediated QS.

摘要

通过逐步将回流比从 50%提高到 500%,在水力停留时间为 12 小时、进水化学需氧量(COD)为 5500mg/L 的条件下,评估了厌氧污泥的性能和微生物社会行为在膨胀颗粒污泥床(EGSB)中的表现。结果表明,回流比为 100%-200%时更有利于 EGSB 的甲烷生成,产甲烷量为 2.4m/m·d。研究发现,酰基高丝氨酸内酯(AHLs)介导的群体感应(QS)可以在厌氧消化过程中平衡各种微生物种群。C-HSL 和 C-HSL 被鉴定为通过刺激蛋白质分泌到细胞外聚合物(EPS)中增强厌氧污泥颗粒化的特定 AHLs。3-氧代-C-HSL 和 3-氧代-C-HSL 被证明可以增强产甲烷作用。本研究基于 AHLs 介导的 QS,为 EGSB 回流比的性能提供了新的视角。

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