Department of Medical, Surgical Sciences and Advanced Technologies "G.F. Ingrassia", Via S. Sofia, 87, 95123, Catania, Italy.
Center of Excellence for the Acceleration of Harm Reduction (CoEHAR), University of Catania, Via S. Sofia, 97, 95123, Catania, Italy.
Regul Toxicol Pharmacol. 2021 Jun;122:104917. doi: 10.1016/j.yrtph.2021.104917. Epub 2021 Mar 18.
Despite the growing numbers of studies on cigarettes and electronic nicotine delivery products (ENDs), no standard assessment of nicotine stability in various matrix post exposure is currently available. The aim of the present study was to evaluate the optimal standard condition to store Cambridge Filter Pads (CFPs) before chemical analysis in order to guarantee the titer of nicotine.We further performed data normalization according to different smoking or vaping runs. Smoke and vapor generated respectively by a reference tobacco cigarette (1R6F) and ENDs under different exposure regimes (ISO, HCI and CRM81) were collected on CFPs as total particulate matter (TPM) and subsequently analyzed for nicotine content. For each exposure, some CFPs were analyzed at time zero, whereas the others were stored under different conditions for nicotine assessment after 30 days. Principal Component Analysis (PCA) showed the best correlation between nicotine on CFPs and TPM for normalization. This study suggests that different exposure regimes and products can affect the preservation of nicotine titer on CFPs while samples storage at -80 °C may prevent the loss of nicotine. Finally, normalization of nicotine with TPM is strongly recommended for regulatory purpose.
尽管越来越多的研究涉及香烟和电子尼古丁输送产品（ENDS），但目前尚无关于各种基质中尼古丁暴露后稳定性的标准评估方法。本研究旨在评估在进行化学分析之前储存剑桥滤片（CFP）的最佳标准条件，以保证尼古丁的效价。我们进一步根据不同的吸烟或蒸气吸入运行情况进行了数据归一化。参考烟草香烟（1R6F）和不同暴露条件（ISO、HCI 和 CRM81）下的 ENDs 分别产生的烟雾和蒸气被收集在 CFP 上作为总颗粒物（TPM），随后分析尼古丁含量。对于每种暴露情况，一些 CFP 在零时进行分析，而其他 CFP 在 30 天后根据不同条件进行尼古丁评估。主成分分析（PCA）表明，尼古丁在 CFP 上的归一化与 TPM 之间具有最佳相关性。本研究表明，不同的暴露条件和产品会影响 CFP 上尼古丁效价的保存，而在-80°C 下储存样品可能会防止尼古丁的损失。最后，强烈建议为监管目的使用 TPM 对尼古丁进行归一化。
