Research Unit of Medical Imaging, Physics and Technology, University of Oulu/Faculty of Medicine, Aapistie 5 A, 90220 Oulu, Finland.
Cancer Research and Translational Medicine Research Unit, University of Oulu, Aapistie 5 A, 90220 Oulu, Finland; Department of Pathology, Oulu University Hospital, Oulu, Finland; Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Finland.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Jun 5;254:119665. doi: 10.1016/j.saa.2021.119665. Epub 2021 Mar 9.
Among skin cancers, melanoma is the lethal form and the leading cause of death in humans. Melanoma begins in melanocytes and is curable at early stages. Thus, early detection and evaluation of its metastatic potential are crucial for effective clinical intervention. Fourier transform infrared (FTIR) spectroscopy has gained considerable attention due to its versatility in detecting biochemical and biological features present in the samples. Changes in these features are used to differentiate between samples at different stages of the disease. Previously, FTIR spectroscopy has been mostly used to distinguish between healthy and diseased conditions. With this study, we aim to discriminate between different melanoma cell lines based on their FTIR spectra. Formalin-fixed paraffin embedded samples from three melanoma cell lines (IPC-298, SK-MEL-30 and COLO-800) were used. Statistically significant differences were observed in the prominent spectral bands of three cell lines along with shifts in peak positions. A partial least square discriminant analysis (PLS-DA) model built for the classification of three cell lines showed an overall accuracy of 92.6% with a sensitivity of 85%, 95.75%, 96.54%, and specificity of 97.80%, 92.14%, 98.64% for the differentiation of IPC-298, SK-MEL-30, and COLO-800, respectively. The results suggest that FTIR spectroscopy can differentiate between different melanoma cell lines and thus potentially characterize the metastatic potential of melanoma.
在皮肤癌中，黑色素瘤是致命形式，也是人类死亡的主要原因。黑色素瘤始于黑色素细胞，在早期阶段是可治愈的。因此，早期检测和评估其转移潜能对于有效的临床干预至关重要。傅里叶变换红外（FTIR）光谱由于能够检测样品中存在的生化和生物学特征而受到广泛关注。这些特征的变化可用于区分疾病不同阶段的样品。以前，FTIR 光谱主要用于区分健康和患病条件。在这项研究中，我们旨在根据 FTIR 光谱区分不同的黑色素瘤细胞系。使用了来自三个黑色素瘤细胞系（IPC-298、SK-MEL-30 和 COLO-800）的福尔马林固定石蜡包埋样本。三个细胞系的突出光谱带以及峰位置的偏移都观察到了统计学上的显著差异。建立的用于三类细胞系分类的偏最小二乘判别分析（PLS-DA）模型显示，总体准确率为 92.6%，对 IPC-298、SK-MEL-30 和 COLO-800 的区分灵敏度分别为 85%、95.75%、96.54%，特异性分别为 97.80%、92.14%、98.64%。结果表明，FTIR 光谱可以区分不同的黑色素瘤细胞系，从而有可能表征黑色素瘤的转移潜能。
