Biophotoics Research Group, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Govt Sadiq College, Women University of Bahawalpur, Bahawalpur, Pakistan.
Cancer Biomark. 2018;23(3):373-380. doi: 10.3233/CBM-181393.
Early diagnosing of skin cancer and investigation of metastatic potential of cancer cells are very important to treat it appropriately. Infrared spectroscopy of biological tissues is an emerging technique which gives the spectral differences between healthy and diseased cells. In this work, we have demonstrated that attenuated total reflectance Fourier transform (ATR-FTIR) spectroscopy can be used in diagnostic of skin cancer and in differentiating metastatic potential of cancer cells. Using IR spectroscopy, we can identify various types of cancer such as basal cell carcinoma, malignant melanoma, nevus and metastatic potential by alternations in hydration level and molecular changes. We examined biopsy of different types of cancer cells to diagnose skin cancer at early stages by using FTIR spectroscopy. To differentiate metastases we examined two human melanoma cells of same patient but at different metastatic potential and two murine melanoma cells with common genetic background but different metastatic potential. Our findings revealed that melanoma changes the permeability of cell membrane and higher metastatic potential is related to the hydration level of cell membrane. Thus, ATR-FTIR spectroscopy is a potential technique to help in early diagnosing of skin cancer and to differentiate different metastatic potentials.
早期诊断皮肤癌和研究癌细胞的转移潜能对于进行适当的治疗非常重要。生物组织的红外光谱是一种新兴技术，可显示健康细胞和病变细胞之间的光谱差异。在这项工作中，我们已经证明，衰减全反射傅里叶变换（ATR-FTIR）光谱可用于皮肤癌的诊断以及癌细胞转移潜能的区分。通过使用红外光谱，我们可以通过水合水平和分子变化的改变来识别各种类型的癌症，例如基底细胞癌、恶性黑色素瘤、痣和转移性。我们通过 FTIR 光谱检查了不同类型的癌细胞活检，以在早期阶段诊断皮肤癌。为了区分转移，我们检查了同一位患者的两种具有相同遗传背景但转移潜能不同的人黑色素瘤细胞，以及两种具有相同遗传背景但转移潜能不同的鼠黑色素瘤细胞。我们的研究结果表明，黑色素瘤改变了细胞膜的通透性，更高的转移潜能与细胞膜的水合水平有关。因此，ATR-FTIR 光谱是一种有潜力的技术，可以帮助早期诊断皮肤癌并区分不同的转移潜能。
