Alsadat Mahmoudian Reihaneh, Lotfi Gharaie Maryam, Abbaszadegan Roya, Forghanifard Mohammad Mahdi, Abbaszadegan Mohammad Reza
Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Division of Physiology, Department of Basic Science, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Iran Biomed J. 2021 Mar 1;25(3):157-68. doi: 10.29252/ibj.25.3.157.
Large intergenic non-coding RNA regulator of reprogramming (LINC-ROR), as a cancer-related Long non-coding RNA, has vital roles in stem cell survival, pluripotency, differentiation, and self-renewal in human embryonic stem cell. However, cancer-related molecular mech¬anisms, its functional roles, and clinical value of LINC-ROR in gastric cancer (GC) remain unclear. In this study, we aimed to investigate probable interplay between LINC-ROR with SALL4 stemness regulator and their role with the development of the disease.
The mRNA expression profile of LINC-ROR and SALL4 was assessed in tumoral and adjacent non-cancerous tissues of GC patients, using quantitative real-time PCR.
Significant LINC-ROR underexpression and SALL4 overexpression were observed in 55.81% and 75.58% (p < 0.0001) of samples, respectively. The expression of LINC-ROR and SALL4 were significantly correlated with each other (p = 0.044). There was an association between the underexpression of LINC-ROR and sex, stage of tumor progression, tumor type, and location of tumor (p < 0.05), and Helicobacter pylori infection with SALL4 expression (p = 0.036). There were also significant correlations between concomitant mRNA expression of SALL4 and LINC-ROR in tumors located at distal noncardiac, positive for H. pylori infection, tumors with invasion into the muscle layer of the stomach, and grade II tumor (p < 0.05).
The clinical results of the SALL4-LINC-ROR association propose a probable functional interaction between these markers in tumor maintenance and aggressiveness. Our study can help to understand one of the mechanisms involved in the progression of gastric cancer through the function of these regulators.
重编程的大型基因间非编码RNA调节因子（LINC-ROR）作为一种与癌症相关的长链非编码RNA，在人类胚胎干细胞的存活、多能性、分化和自我更新中起着至关重要的作用。然而，LINC-ROR在胃癌（GC）中的癌症相关分子机制、功能作用和临床价值仍不清楚。在本研究中，我们旨在研究LINC-ROR与干性调节因子SALL4之间可能的相互作用及其在疾病发展中的作用。
采用定量实时PCR技术，评估GC患者肿瘤组织及癌旁非癌组织中LINC-ROR和SALL4的mRNA表达谱。
分别在55.81%和75.58%的样本中观察到LINC-ROR显著低表达和SALL4过表达（p < 0.0001）。LINC-ROR和SALL4的表达彼此显著相关（p = 0.044）。LINC-ROR低表达与性别、肿瘤进展阶段、肿瘤类型和肿瘤位置之间存在关联（p < 0.05），幽门螺杆菌感染与SALL4表达之间存在关联（p = 0.036）。在位于非贲门远端、幽门螺杆菌感染阳性、侵犯胃肌层的肿瘤和II级肿瘤中，SALL4和LINC-ROR的mRNA伴随表达之间也存在显著相关性（p < 0.05）。
SALL4-LINC-ROR关联的临床结果表明，这些标志物在肿瘤维持和侵袭性方面可能存在功能相互作用。我们的研究有助于通过这些调节因子的功能来理解参与胃癌进展的机制之一。
