Rezaei Majdaddin, Emadi-Baygi Modjtaba, Hoffmann Michèle J, Schulz Wolfgang A, Nikpour Parvaneh
Pediatric Inherited Diseases Research Center, Research Institute for Primordial Prevention of Non-communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Tumour Biol. 2016 Feb;37(2):1763-9. doi: 10.1007/s13277-015-3933-x. Epub 2015 Aug 28.
According to GLOBOCAN 2012, the worldwide burden of cancer increased and is expected to worsen within the next decades. Therefore, universal combat against cancer will not succeed with treatment solely; effective prevention and early detection are urgently needed to tackle the cancer crisis. Emerging data demonstrate that long non-coding RNAs are involved in numerous biological and pathological processes like development and differentiation and in a variety of human diseases including cancer. Located at 18q21, LINC-ROR (regulator of reprogramming) is a modulator of ESCs maintenance and hypoxia-signaling pathways in hepatocellular cancer cells. The aim of this study was to examine the expression of LINC-ROR in various cell lines and representative samples of human cancers by quantitative real-time RT-PCR to provide a snapshot on how LINC-ROR expression may be deregulated in cancer. More than 30 cell lines and 112 patient specimens from various tissues were assessed for relative expression of LINC-ROR. Our results revealed that the expression of LINC-ROR was lower in all somatic cancer cell lines compared to stem cells or cells with stem cell-like capabilities, like the embryonic carcinoma cell line, NTERA-2. In tissues, expression patterns vary, but some cancerous tissues displayed increased LINC-ROR expression compared to corresponding normal tissues. Thus, we hypothesize that LINC-ROR may have a key function in a subpopulation of cells from the tumor bulk, i.e., the cancer stem cells associated with specific properties including resistance to adverse environmental conditions.
根据《2012年全球癌症统计》，全球癌症负担有所增加，预计在未来几十年内还会恶化。因此，仅靠治疗无法成功实现全球抗癌目标；迫切需要有效的预防和早期检测来应对癌症危机。新出现的数据表明，长链非编码RNA参与了许多生物和病理过程，如发育和分化，以及包括癌症在内的多种人类疾病。LINC-ROR（重编程调节因子）位于18q21，是肝癌细胞中胚胎干细胞维持和缺氧信号通路的调节因子。本研究的目的是通过定量实时逆转录聚合酶链反应检测LINC-ROR在各种细胞系和人类癌症代表性样本中的表达，以了解LINC-ROR在癌症中可能是如何失调的。对30多个细胞系和来自不同组织的112份患者标本进行了LINC-ROR相对表达的评估。我们的结果显示，与干细胞或具有干细胞样能力的细胞（如胚胎癌细胞系NTERA-2）相比，所有体细胞癌细胞系中LINC-ROR的表达均较低。在组织中，表达模式各不相同，但与相应正常组织相比，一些癌组织中LINC-ROR的表达有所增加。因此，我们推测LINC-ROR可能在肿瘤主体的一个细胞亚群中发挥关键作用，即与包括对不利环境条件的抗性在内的特定特性相关的癌症干细胞。
