Hu Xiaowen, Saravanakumar Kandasamy, Jin Tieyan, Wang Myeong-Hyeon
Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, 200-701 South Korea.
Department of Food Science and Engineering, College of Agricultural, Yanbian University, Yanji, Jilin China.
J Food Sci Technol. 2021 Apr;58(4):1499-1510. doi: 10.1007/s13197-020-04663-4. Epub 2020 Sep 5.
The present work examined the biomedical value of red and yellow bell pepper extracts (YME and RME) in terms of antioxidant, antibacterial and anticancer activities by in vitro and virtual studies. The yield of extract was 3.49% for RME and 2.92% for YME. The level of total phenols and total flavonoids significantly varied between the type of extracts, and it was higher in RME than that in YME. The extracts showed promising DPPH and ABTS free radical scavenging rates. The extracts showed an excellent antibacterial activity. The minimal inhibitory concentration (MIC) of RME was 0.20 mg mL for , 0.30 mg mL for , 0.50 mg mL for and 0.60 mg mL and for , while the MIC of YME was 0.40 mg mL for 0.40 mg mL for , 0.50 mg mL for , and 0.60 mg mL for . TEM results demonstrated the cellular damage induced by RME in biofilm. The RME did not show any cytotoxicity in normal NIH3T3 cells, but at 125 μg mL did a strong cytotoxicity in human lung cancer cell line A549 as evident by cytotoxicity assay, ROS and AO/EB staining. The virtual biological examination indicated that β-carotene from RME was a potential compound with higher docking energy against both targeted enzymes and proteins as - 14.30 for LpxC and - 15.59 for survivin. Therefore, it is recommended that RME is a better functional food with novel biomedical properties and it deserves further evaluation for its the novel molecules against multidrug resistant pathogens.
本研究通过体外和虚拟研究，考察了红甜椒提取物（RME）和黄甜椒提取物（YME）在抗氧化、抗菌和抗癌活性方面的生物医学价值。RME的提取物得率为3.49%，YME的提取物得率为2.92%。提取物类型之间的总酚和总黄酮水平存在显著差异，RME中的含量高于YME。提取物表现出良好的DPPH和ABTS自由基清除率。提取物表现出优异的抗菌活性。RME对金黄色葡萄球菌的最低抑菌浓度（MIC）为0.20 mg/mL，对大肠杆菌为0.30 mg/mL，对枯草芽孢杆菌为0.50 mg/mL，对白色念珠菌为0.60 mg/mL，而YME对金黄色葡萄球菌的MIC为0.40 mg/mL，对大肠杆菌为0.40 mg/mL，对枯草芽孢杆菌为0.50 mg/mL，对白色念珠菌为0.60 mg/mL。透射电镜结果表明RME对金黄色葡萄球菌生物膜有细胞损伤作用。RME在正常NIH3T3细胞中未表现出任何细胞毒性，但在125 μg/mL时对人肺癌细胞系A549有很强的细胞毒性，细胞毒性试验、活性氧和AO/EB染色均有明显表现。虚拟生物学检查表明，RME中的β-胡萝卜素是一种潜在的化合物，对两种靶向酶和蛋白质具有较高的对接能量，对LpxC为-14.30，对生存素为-15.59。因此，建议RME是一种具有新型生物医学特性的更好的功能性食品，其针对多重耐药病原体的新型分子值得进一步评估。
