Foroughi-Parvar Faeze, Sarkari Bahador, Asgari Qasem, Hatam Gholamreza
Department of Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
J Parasit Dis. 2021 Mar;45(1):279-284. doi: 10.1007/s12639-020-01316-3. Epub 2020 Nov 10.
Although canids are regarded as major reservoir hosts for , feline leishmaniasis are reported sporadically from different endemic foci of Mediterranean visceral leishmaniasis (VL). Despite the risk of parasite transmission between human and other animals, most of the studies are limited to dogs and few studies are focused to investigate sp. among other mammals. This project was aimed to detect antibodies of cats in two VL endemic regions of Iran by Fucose Mannose Ligand (FML) and soluble antigen (SLA) ELISA. Forty nine stray cats of different age and sex, from Fars and Ardabil provinces (two VL endemic loci of Iran) were sampled, then tested for by FML and SLA-ELISA. Sixteen percent (8/49) of cat sera were reported positive by FML-ELISA. SLA-ELISA showed 18.3% (9/48) positive cases in cats. Sensitivity of FML-ELISA was calculated 57% and SLA ELISA 25%. Specificity of FML and SLA ELISA were assessed 78% and 68% respectively. Kappa coefficient of agreement between FML and SLA-ELISA was detected on 0.45. As feline leishmaniasis could be a potential risk in endemic areas, FML-ELISA could be considered as an appropriate examination to detect leishmaniasis in cats.
尽管犬科动物被视为利什曼原虫的主要储存宿主，但在地中海内脏利什曼病（VL）的不同流行病灶中，猫利什曼病也有零星报道。尽管存在人和其他动物之间寄生虫传播的风险，但大多数研究仅限于狗，很少有研究专注于调查其他哺乳动物中的利什曼原虫。本项目旨在通过岩藻糖甘露糖配体（FML）和可溶性利什曼原虫抗原（SLA）酶联免疫吸附测定（ELISA）检测伊朗两个VL流行地区猫的利什曼原虫抗体。对来自法尔斯省和阿尔达比勒省（伊朗的两个VL流行地点）的49只不同年龄和性别的流浪猫进行采样，然后通过FML和SLA-ELISA检测利什曼原虫。FML-ELISA报告16%（8/49）的猫血清呈阳性。SLA-ELISA显示猫中有18.3%（9/48）的阳性病例。计算得出FML-ELISA的敏感性为57%，SLA ELISA为25%。FML和SLA ELISA的特异性分别评估为78%和68%。FML和SLA-ELISA之间的kappa一致性系数检测为0.45。由于猫利什曼病在流行地区可能是一个潜在风险，FML-ELISA可被视为检测猫利什曼病的合适检测方法。
