Li Yingchen, Liu Lin, Yang Jun, Yang Qing
School of Bioengineering, Dalian University of Technology, Dalian, Liaoning 116024, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection and Shenzhen Agricultural Genome Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
J Pestic Sci. 2021 Feb 20;46(1):43-52. doi: 10.1584/jpestics.D20-085.
Chitin deacetylase (CDA) is a key enzyme involved in the modification of chitin and plays critical roles in molting and pupation, which catalyzes the removal of acetyl groups from -acetyl-D-glucosamine residues in chitin to form chitosan and release acetic acid. Defects in the CDA genes or their expression may lead to stunted insect development and even death. Therefore, CDA can be used as a potential pest control target. However, there are no effective pesticides known to target CDA. Although there has been some exciting research progress on bacterial or fungal CDAs, insect CDA characteristics are less understood. This review summarizes the current understanding of insect CDAs, especially very recent advances in our understanding of crystal structures and the catalytic mechanism. Progress in developing small-molecule CDA inhibitors is also summarized. We hope the information included in this review will help facilitate new pesticide development through a novel action mode, such as targeting CDA.
几丁质脱乙酰酶（CDA）是参与几丁质修饰的关键酶，在蜕皮和化蛹过程中发挥着关键作用，它催化几丁质中β-乙酰-D-葡萄糖胺残基上的乙酰基去除，形成壳聚糖并释放乙酸。CDA基因或其表达的缺陷可能导致昆虫发育迟缓甚至死亡。因此，CDA可作为潜在的害虫防治靶点。然而，目前尚无已知针对CDA的有效农药。尽管在细菌或真菌CDA方面已有一些令人兴奋的研究进展，但对昆虫CDA的特性了解较少。本文综述了目前对昆虫CDA的认识，特别是对其晶体结构和催化机制的最新研究进展。同时也总结了开发小分子CDA抑制剂的进展。我们希望本综述所包含的信息将有助于通过靶向CDA等新型作用模式推动新型农药的开发。
