Li Xue, Wang Xiaoxuan, Luan Qing-Xian
Department of Periodontology, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing 100081, P.R. China.
Exp Ther Med. 2021 May;21(5):417. doi: 10.3892/etm.2021.9861. Epub 2021 Feb 25.
The present study aimed to investigate whether gingival fibroblasts (GFs) of patients with aggressive periodontitis (AgP) are more sensitive to lipopolysaccharide (LPS) stimulation than GFs of control subjects. AgP causes rapid periodontal destruction, including the production of cytokines [i.e. interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α] and matrix metalloproteinases (MMP)-1, -3 and -9 in AgP GFs. LPS upregulates IL-1β, IL-6, TNF-α, MMP-1, MMP-3, MMP-9 and mitochondrial reactive oxygen species (mtROS). Fibroblasts are known to be associated with immune responses to bacterial virulence factors, but the precise mechanisms underlying this severe periodontal disease are unclear. In the present study, primary human GFs of four patients with AgP and four healthy subjects were challenged with LPS from (). The generation of mtROS in GFs was assessed using MitoSOX Red. The expression of genes encoding inflammatory cytokines and MMPs in GFs was analyzed using reverse transcription-quantitative polymerase chain reaction, and the expression of proteins was analyzed using ELISA and Western blotting. Human GFs of patients with AgP exhibited higher levels of mtROS, and higher mRNA and protein expression levels of proinflammatory cytokines, including IL-1β, IL-6, MMP-1, MMP-3 and MMP-9 compared with healthy human GFs following stimulation with LPS from . In the present study, it was demonstrated that GFs of patients with AgP display hyperreactivity when challenged with LPS.
本研究旨在调查侵袭性牙周炎（AgP）患者的牙龈成纤维细胞（GFs）是否比对照受试者的GFs对脂多糖（LPS）刺激更敏感。AgP会导致快速的牙周组织破坏，包括AgP GFs中细胞因子[即白细胞介素（IL）-1β、IL-6和肿瘤坏死因子（TNF）-α]以及基质金属蛋白酶（MMP）-1、-3和-9的产生。LPS可上调IL-1β、IL-6、TNF-α、MMP-1、MMP-3、MMP-9和线粒体活性氧（mtROS）。已知成纤维细胞与对细菌毒力因子的免疫反应有关，但这种严重牙周疾病的具体机制尚不清楚。在本研究中，对4例AgP患者和4例健康受试者的原代人GFs用来自（）的LPS进行刺激。使用MitoSOX Red评估GFs中mtROS的产生。使用逆转录定量聚合酶链反应分析GFs中编码炎性细胞因子和MMPs的基因表达，并使用ELISA和蛋白质印迹法分析蛋白质表达。与用来自（）的LPS刺激后的健康人GFs相比，AgP患者的人GFs表现出更高水平的mtROS，以及促炎细胞因子（包括IL-1β、IL-6、MMP-1、MMP-3和MMP-9）更高的mRNA和蛋白质表达水平。在本研究中，证明了AgP患者的GFs在用LPS刺激时表现出高反应性。
