Chang Tie, Moses Oyawale Adetunji, Tian Conghui, Wang Hai, Song Li, Zhao Gang
Department of Electronic Science and Technology University of Science and Technology of China No. 96 Road Jinzhai Hefei Anhui 230027 China.
National Synchrotron Radiation Laboratory CAS Center for Excellence in Nanoscience University of Science and Technology of China Hefei Anhui 230029 China.
Adv Sci (Weinh). 2021 Jan 29;8(6):2003387. doi: 10.1002/advs.202003387. eCollection 2021 Mar.
Despite recent advances in controlling ice formation and growth, it remains a challenge to design anti-icing materials in various fields from atmospheric to biological cryopreservation. Herein, tungsten diselenide (WSe)-polyvinyl pyrrolidone (PVP) nanoparticles (NPs) are synthesized through one-step solvothermal route. The WSe-PVP NPs show synergetic ice regulation ability both in the freezing and thawing processes. Molecularly speaking, PVP containing amides group can form hydrogen bonds with water molecules. At a macro level, the WSe-PVP NPs show adsorption-inhibition and photothermal conversation effects to synergistically restrict ice growth. Meanwhile, WSe-PVP NPs are for the first time used for the cryopreservation of human umbilical vein endothelial cell (HUVEC)-laden constructs based on rapid freezing with low concentrations of cryoprotectants (CPAs), the experimental results indicate that a minimal concentration (0.5 mg mL) of WSe-PVP NPs can increase the viabilities of HUVECs in the constructs post cryopreservation (from 55.8% to 83.4%) and the cryopreserved constructs can also keep good condition within 7 days. Therefore, this work provides a novel strategy to synergistically suppress the formation and growth of the ice crystalsfor the cryopreservation of cells, tissues, or organs.
尽管在控制冰的形成和生长方面取得了最新进展，但从大气到生物冷冻保存等各个领域设计防冰材料仍然是一项挑战。在此，通过一步溶剂热法合成了二硒化钨（WSe）-聚乙烯吡咯烷酮（PVP）纳米颗粒（NPs）。WSe-PVP NPs在冷冻和解冻过程中均表现出协同的冰调控能力。从分子层面来讲，含有酰胺基团的PVP可与水分子形成氢键。在宏观层面，WSe-PVP NPs表现出吸附抑制和光热转换效应，以协同限制冰的生长。同时，WSe-PVP NPs首次用于基于低浓度冷冻保护剂（CPAs）快速冷冻的载有人脐静脉内皮细胞（HUVEC）构建体的冷冻保存，实验结果表明，最低浓度（0.5 mg/mL）的WSe-PVP NPs可提高冷冻保存后构建体中HUVECs的活力（从55.8%提高到83.4%），且冷冻保存的构建体在7天内也能保持良好状态。因此，这项工作为协同抑制冰晶形成和生长以用于细胞、组织或器官的冷冻保存提供了一种新策略。
