School of Medical Imaging, Bengbu Medical College, Bengbu, China.
Department of Electronic Engineering and Information Science, University of Science and Technology of China, Hefei, China.
Biopreserv Biobank. 2022 Aug;20(4):311-316. doi: 10.1089/bio.2022.0111.
Long-term cryopreservation of human umbilical vein endothelial cells (HUVECs) is important and beneficial for a variety of biomedical research and applications. In this study, we investigated HUVEC's cryobiological characteristics and parameters that are indispensable for predicting and determining an optimal cooling rate to prevent lethal intracellular ice formation (IIF) and severe cell dehydration during the cryopreservation processes. The parameters include cell membrane hydraulic conductivity (i.e., cell membrane water permeability), , cell membrane water permeability activation energy, , and osmotically inactive volume of a cell . Cryomicroscopy was used to study the IIF phenomena and cell volume excursion at various cooling rates, 1, 10, and 20°C/min, respectively, based on which the cryobiological parameters were determined using biophysical and mathematical models. Results from this research work laid an important cryobiological foundation for the optimization of HUVEC's cryopreservation conditions.
长期冷冻保存人脐静脉内皮细胞（HUVECs）对于各种生物医学研究和应用非常重要和有益。在这项研究中，我们研究了 HUVEC 的低温生物学特性和参数，这些参数对于预测和确定最佳冷却速率以防止致命的细胞内冰形成（IIF）和冷冻保存过程中严重的细胞脱水是必不可少的。这些参数包括细胞膜水力传导性（即细胞膜水通透性）、细胞膜水通透性激活能、细胞不可渗透体积。基于此，我们分别使用冷冻显微镜研究了在 1°C/min、10°C/min 和 20°C/min 等不同冷却速率下的 IIF 现象和细胞体积偏移，然后使用生物物理和数学模型来确定低温生物学参数。这项研究工作为优化 HUVEC 的冷冻保存条件奠定了重要的低温生物学基础。
