Raji M A, Kazeem H M, Magyigbe K A, Ahmed A O, Lawal D N, Raufu I A
Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Ilorin, Kwara State, Nigeria.
Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Ahmadu Bello University Zaria, Nigeria.
Int J Food Sci. 2021 Mar 2;2021:8872137. doi: 10.1155/2021/8872137. eCollection 2021.
Salmonellosis is one of the most common and widely distributed food-borne diseases, and the presence of antimicrobial-resistant in poultry and poultry products is a global public health problem. Therefore, a cross-sectional study was conducted from November 2016 to July 2017 with an aim of determining the isolation rates of species from the intestinal contents of slaughtered chickens, the most common serotypes that invade and colonize the tissues of chickens in Ilorin, and the susceptibilities of the isolated species to commonly used antibiotics. Four hundred samples of intestinal contents from apparently healthy slaughtered chickens and one hundred ready-to-eat chicken gizzards in Ilorin, Kwara State, were examined for the presence of and their serotypes. were isolated and identified according to the techniques recommended by the World Health Organization: preenrichment, selective plating, biochemical testing, and serotyping. A total number of forty-three (43) isolates consisting of 33 from intestinal contents and 10 from ready-to-eat chicken gizzards were isolated and identified. There was an overall prevalence rate of 8.6% (43/500), and the isolates were distributed as follows: gizzard, 2% ( = 10) and intestinal contents, 6.6% ( = 33). The predominant serovars were subsp. serovar 45: d: 1, 7 (16) and Haifa (5). All ready-to-eat chicken gizzards were associated with subsp. serovar 45: d: 1, 7 (5). The from intestinal contents belong to subsp. serovars 45: d: 1, 7 (11) and . Haifa (5). species isolated were 100% resistant to ciprofloxacin, ampicillin, and ceftazidime. This is followed by cloxacillin (81%), tetracycline (75%), and sulfamethoxazole (67%). The isolates were, however, 100% sensitive to enrofloxacin, 74% to streptomycin, and 72% to gentamycin antibiotics. The most common serotype was subsp. a serovar 45: d: 1, 7. All the twenty five serovars consisting of twenty-one serotypes ( = 21), two of the that could not be cultured after enrichment, and the two that were contaminated with possessed the virulence genes of and . The subsp. serovar 45: d: 1, 7 and . Haifa possess virulence genes so they are potentially virulent for humans in this area. The national and local health authorities in Nigeria should improve hygiene measures especially at retail slaughter markets to reduce salmonellosis which is one of the most important food-borne diseases in humans.
沙门氏菌病是最常见且分布广泛的食源性疾病之一，家禽及家禽产品中存在的抗菌药物耐药性是一个全球性的公共卫生问题。因此，于2016年11月至2017年7月开展了一项横断面研究，旨在确定屠宰鸡肠道内容物中沙门氏菌的分离率、伊洛林地区侵入并定植于鸡组织的最常见血清型，以及分离出的菌株对常用抗生素的敏感性。对来自夸拉州伊洛林地区看似健康的屠宰鸡的400份肠道内容物样本和100份即食鸡胗进行了沙门氏菌及其血清型的检测。按照世界卫生组织推荐的技术进行沙门氏菌的分离和鉴定：预富集、选择性平板培养、生化试验和血清分型。共分离并鉴定出43株沙门氏菌，其中33株来自肠道内容物，10株来自即食鸡胗。总体患病率为8.6%（43/500），分离株分布如下：鸡胗，2%（n = 10）；肠道内容物，6.6%（n = 33）。主要血清型为肠炎沙门氏菌亚种肠炎血清型45: d: 1, 7（16株）和海法沙门氏菌（5株）。所有即食鸡胗均与肠炎沙门氏菌亚种肠炎血清型45: d: 1, 7相关（5株）。来自肠道内容物的沙门氏菌属于肠炎沙门氏菌亚种血清型45: d: 1, 7（11株）和海法沙门氏菌（5株）。分离出的沙门氏菌对环丙沙星、氨苄西林和头孢他啶的耐药率为100%。其次是氯唑西林（81%）、四环素（75%）和磺胺甲恶唑（67%）。然而，分离出的沙门氏菌对恩诺沙星的敏感率为100%对链霉素为74%，对庆大霉素为72%。最常见的血清型是肠炎沙门氏菌亚种血清型45: d: 1, 7。所有25株沙门氏菌血清型包括21种血清型（n = 21）、富集后无法培养的2株沙门氏菌以及2株被污染的沙门氏菌均具有沙门氏菌和肠炎沙门氏菌的毒力基因。肠炎沙门氏菌亚种肠炎血清型45: d: 1, 7和海法沙门氏菌具有毒力基因，因此在该地区对人类具有潜在致病性。尼日利亚国家和地方卫生当局应改善卫生措施，尤其是在零售屠宰市场，以减少沙门氏菌病，沙门氏菌病是人类最重要的食源性疾病之一。
