通过组织蛋白质组学分析绘制肝损伤的新型生物标志物

Mapping Novel Biomarkers of Liver Injury by Tissue Proteomic Analysis.

作者信息

Yang Bo, Zhang Jianan, Sun Le, Huang Tingmei, Kong Yaqi, Li Lei, Sun Zhengwang, Yin Mengchen, Li Xiaotao

机构信息

Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai 200241, China.

Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 201210, China.

出版信息

ACS Omega. 2021 Mar 2;6(10):7127-7138. doi: 10.1021/acsomega.1c00152. eCollection 2021 Mar 16.

Liver damage is a dynamic process, and evaluation of liver injury degree is the key step in disease diagnosis. However, few common markers among different types of liver injury have been reported. Herein, we generated three liver injury mouse models, including Con A-induced, CCl-injected, and subjected bile duct ligation mouse models, to simulate different types of liver damage in humans and then performed a label-free mass spectrometry to identify differentially expressed proteins in liver tissues. Interestingly, two proteins, G3BP and ABCC6, were conserved regulated in different liver injury models and are proposed to be biomarkers in liver injury, with G3BP upregulated and ABCC6 downregulated. Overall, our study identified two novel biomarkers of liver injury, and they might be used as potential drug targets of liver damage research studies.

肝损伤是一个动态过程，评估肝损伤程度是疾病诊断的关键步骤。然而，不同类型肝损伤中常见的标志物报道较少。在此，我们构建了三种肝损伤小鼠模型，包括刀豆蛋白A诱导的、四氯化碳注射的和胆管结扎小鼠模型，以模拟人类不同类型的肝损伤，然后进行无标记质谱分析以鉴定肝组织中差异表达的蛋白质。有趣的是，两种蛋白质，G3BP和ABCC6，在不同的肝损伤模型中受到保守调控，被认为是肝损伤的生物标志物，G3BP上调而ABCC6下调。总体而言，我们的研究鉴定出两种新型肝损伤生物标志物，它们可能用作肝损伤研究的潜在药物靶点。