Yuan Jifeng, Mo Qiwen, Fan Cong
State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, China.
ACS Omega. 2021 Mar 2;6(10):7175-7180. doi: 10.1021/acsomega.1c00339. eCollection 2021 Mar 16.
Promoters that play an essential role in the gene regulation are of particular interest to the synthetic biology communities. Recent advances in high-throughput DNA sequencing have greatly increased the breadth of new genetic parts. The development of promoters with broad host properties could enable rapid phenotyping of genetic constructs in different hosts. In this study, we discovered that the GAL1/10 bidirectional promoter from could be used for shuttle expression in . Further investigation revealed that the GAL1/10 bidirectional promoter is subjected to catabolite repression in . We next constructed a set of Golden-Gate assembly vectors for shuttle expression between and . The utility of shuttle vectors was demonstrated for rapid phenotyping of a multigene pathway for cinnamyl alcohol production. Taken together, our work opens a new avenue for the future development of broad host expression systems between prokaryotic and eukaryotic hosts.
在基因调控中发挥关键作用的启动子尤其受到合成生物学界的关注。高通量DNA测序的最新进展极大地拓宽了新遗传元件的范围。具有广泛宿主特性的启动子的开发能够实现不同宿主中遗传构建体的快速表型分析。在本研究中，我们发现来自[具体来源]的GAL1/10双向启动子可用于在[具体宿主]中进行穿梭表达。进一步研究表明，GAL1/10双向启动子在[具体宿主]中受到分解代谢物阻遏。接下来，我们构建了一组用于在[具体宿主1]和[具体宿主2]之间进行穿梭表达的金门组装载体。穿梭载体的实用性通过对肉桂醇生产多基因途径的快速表型分析得到了证明。综上所述，我们的工作为原核和真核宿主之间广泛宿主表达系统的未来发展开辟了一条新途径。
