del Aguila C, Cuellar C, Guillén J L
Departamento de Parasitología, Facultad de Farmacia, Universidad Computense de Madrid, Spain.
Parasite Immunol. 1988 Mar;10(2):237-41. doi: 10.1111/j.1365-3024.1988.tb00217.x.
Five monoclonal antibodies against the excretory/secretory (E/S) antigen of Toxocara canis were obtained and characterized. Immunoprecipitating activity was demonstrated in an in-vitro microprecipitating assay using live T. canis larvae. Their capacity to kill larvae was also shown in an in-vitro assay. Two zones of reactivity were observed in 7.5 and 12.5% SDS-PAGE (177-77 kD, 43-15 kD) of immunoprecipitates of human and mouse positive polyclonal antisera. The murine monoclonal antibodies showed a common pattern of reactivity with the proteins in the 177-77 kD range.
获得并鉴定了五种针对犬弓首蛔虫排泄/分泌（E/S）抗原的单克隆抗体。使用活的犬弓首蛔虫幼虫在体外微沉淀试验中证明了免疫沉淀活性。在体外试验中也显示了它们杀死幼虫的能力。在人和小鼠阳性多克隆抗血清的免疫沉淀物的7.5%和12.5%十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE，177-77kD，43-15kD）中观察到两个反应区。鼠单克隆抗体与177-77kD范围内的蛋白质表现出共同的反应模式。
