Badley J E, Grieve R B, Rockey J H, Glickman L T
Parasite Immunol. 1987 Jan;9(1):133-43. doi: 10.1111/j.1365-3024.1987.tb00494.x.
The participation of Toxocara canis larval excretory-secretory antigens in immune-mediated adherence was determined in vitro. Adsorption of immune sera with excretory-secretory antigens removed some complement components, removed IgG antibody directed against larval surfaces, and abrogated all adherence observed with untreated immune serum. At least four antigens could be implicated in adherence, by Western blot analysis of adherence mediating sera. Scanning and transmission electron microscopic examination of larval-eosinophil interactions revealed that eosinophils adhered to a membranous sheath-like layer that was frequently detached from the larval epicuticle. The layers appeared to be composed of surface antigens and antibody, and may provide larvae with protection against antibody and eosinophil toxins by preventing their contact with the epicuticle. The release of surface antigens also may be important in allowing larvae to evade the host's immune response by facilitating the removal of antibody and eosinophils from the larval surface.
在体外确定了犬弓首蛔虫幼虫排泄-分泌抗原在免疫介导黏附中的参与情况。用排泄-分泌抗原吸附免疫血清可去除一些补体成分，去除针对幼虫表面的IgG抗体，并消除未处理免疫血清所观察到的所有黏附现象。通过对介导黏附血清的蛋白质印迹分析，至少有四种抗原可能与黏附有关。对幼虫-嗜酸性粒细胞相互作用的扫描和透射电子显微镜检查显示，嗜酸性粒细胞黏附于一层膜状鞘样层，该层常与幼虫表皮分离。这些层似乎由表面抗原和抗体组成，并可能通过阻止抗体和嗜酸性粒细胞毒素与表皮接触，为幼虫提供针对它们的保护。表面抗原的释放对于幼虫通过促进抗体和嗜酸性粒细胞从幼虫表面的清除来逃避宿主免疫反应也可能很重要。
