Williamson H J, Allardyce R A, Clemett R S, Hidajat R R
Department of Surgery, Christchurch Hospital, New Zealand.
Parasite Immunol. 1990 Mar;12(2):175-87. doi: 10.1111/j.1365-3024.1990.tb00946.x.
Infective larvae of Toxocara canis are well suited for studies of nematode antigen expression in vitro. Larvae were labelled with 3H-glucosamine, an approach permitting dual analysis of antigen quantity and composition. Their excretory/secretory (E/S) glycoproteins were efficiently labelled and antigen identity confirmed by immunoprecipitation, SDS-PAGE and fluorography. Compartmental analysis revealed that common components of Mr 100-120 kD were present in somatic, surface and soluble material. The application of biosynthetic labelling and compartmental analysis of parasite responses in vitro to antibody, complement and neutrophils was tested. Results indicated that test larvae in vitro respond by quantitative rather than qualitative changes in antigen production. Specifically, human serum was shown to raise, and neutrophils depress, the rate of antigen release. The implications of these findings for establishing an in-vitro model for analysis of host/parasite reciprocal adaptive responses are discussed.
犬弓首蛔虫的感染性幼虫非常适合用于线虫抗原体外表达的研究。幼虫用³H - 葡糖胺进行标记，这种方法可对偶联分析抗原数量和组成。它们的排泄/分泌（E/S）糖蛋白被有效标记，通过免疫沉淀、SDS - 聚丙烯酰胺凝胶电泳和荧光自显影确认了抗原的同一性。区室分析表明，分子量为100 - 120 kD的常见成分存在于体细胞、表面和可溶性物质中。测试了生物合成标记和寄生虫体外对抗体、补体和中性粒细胞反应的区室分析的应用。结果表明，体外测试的幼虫通过抗原产生的定量而非定性变化做出反应。具体而言，人血清可提高，而中性粒细胞可降低抗原释放速率。讨论了这些发现对于建立用于分析宿主/寄生虫相互适应性反应的体外模型的意义。
