School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, China.
Biomed Chromatogr. 2021 Aug;35(8):e5120. doi: 10.1002/bmc.5120. Epub 2021 Apr 22.
Knoxiae Radix (HDJ, usually used after being processed into CHDJ) is a traditional Chinese herbal medicine that has been recorded in the Chinese Pharmacopoeia for many years. It is said that Glycyrrhizae Radix et Rhizoma (GC) is incompatible with HDJ, but this is unproven. In this work, nontargeted metabolomics experiments were performed on rats to evaluate the effect of the combination of the two herbals. For this, we conducted a 28-day administration in rats. The plasma, urine and kidney tissues were collected for a metabolomics study based on H NMR and LC-MS. The OPLS-DA method was used to screen biomarkers. In addition, the KEGG Pathway database and MetaboAnalyst were used to find metabolic pathways. Twenty-two significant metabolites were identified. These metabolites were related to many metabolic pathways such as amino acid metabolism, synthesis and degradation of ketone bodies. Significant changes in urine creatinine levels revealed that CHDJ is nephrotoxic. When the GC-CHDJ mass ratio was 1, the toxicity was strengthened; when the GC-CHDJ' mass ratio was 3, the toxicity was alleviated. This is the first time that a metabolomics approach has been used to investigate the incompatibility of GC-CHDJ.
款冬花（HDJ，通常经过炮制后成为 CHDJ）是一种传统的中草药，多年来一直被收录在中国药典中。据说甘草（GC）与 HDJ 不相容，但这尚未得到证实。在这项工作中，我们对大鼠进行了非靶向代谢组学实验，以评估这两种草药组合的效果。为此，我们对大鼠进行了为期 28 天的给药。收集血浆、尿液和肾脏组织，基于 1 H NMR 和 LC-MS 进行代谢组学研究。采用 OPLS-DA 方法筛选生物标志物。此外，KEGG 通路数据库和 MetaboAnalyst 用于寻找代谢途径。鉴定出 22 个显著代谢物。这些代谢物与氨基酸代谢、酮体的合成和降解等多种代谢途径有关。尿液肌酐水平的显著变化表明 CHDJ 具有肾毒性。当 GC-CHDJ 质量比为 1 时，毒性增强；当 GC-CHDJ'质量比为 3 时，毒性减轻。这是首次采用代谢组学方法研究 GC-CHDJ 的不相容性。
