Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Canadian Food Inspection Agency, Saskatoon, Saskatchewan, Canada.
J Vet Intern Med. 2021 May;35(3):1389-1396. doi: 10.1111/jvim.16103. Epub 2021 Mar 10.
Urinary tract infections (UTIs) are common in dogs and can be caused by multidrug-resistant Escherichia coli (E coli).
To describe the frequency and mechanisms of antimicrobial resistance (AMR) among E coli causing UTIs in dogs in Western Canada during a 4-year surveillance period.
Urine from 516 dogs.
From November 2014 to 2018, 516 nonduplicate E coli isolates from the urine of dogs were collected from a diagnostic laboratory. Susceptibility testing was determined for a panel of 14 antimicrobials belonging to 7 drug classes. Resistant isolates were screened for the presence of extended-spectrum beta-lactamases (ESBLs), AmpC β-lactamases, and plasmid-mediated quinolone resistance (PMQR) genes. Epidemiological relationships were assessed by MLST.
80.2% (414/516) of isolates were susceptible to all antimicrobials tested. There was no significant increase in the proportion of isolates resistant to any of the tested antimicrobials during the study period. Resistance to ampicillin was the most common (14.9%, 77/516). Overall, 12 isolates had bla -type AmpC β-lactamases, and 7 produced CTX-M-type ESBLs. A single isolate had the aac(6')-Ib-cr PMQR gene. The qnr and qepA determinants were not detected. A single isolate belonging to the pandemic lineage ST131 was identified.
Escherichia coli isolated from the urine of dogs in our region remain susceptible to first-line therapies, though resistance, particularly to the aminopenicillins, warrants monitoring. This is the first description of E coli ST131 from a companion animal in Canada.
尿路感染（UTIs）在狗中很常见，可由多药耐药性大肠杆菌（E coli）引起。
描述在加拿大西部的一个 4 年监测期间，引起犬 UTIs 的大肠杆菌的抗菌药物耐药性（AMR）的频率和机制。
516 只狗的尿液。
2014 年 11 月至 2018 年，从一家诊断实验室收集了 516 例非重复犬大肠杆菌尿分离株。对属于 7 个药物类别的 14 种抗菌药物的药敏试验进行了测定。筛选耐药分离株是否存在超广谱β-内酰胺酶（ESBLs）、AmpC β-内酰胺酶和质粒介导的喹诺酮耐药（PMQR）基因。通过 MLST 评估流行病学关系。
80.2%（414/516）的分离株对所有测试的抗菌药物均敏感。在研究期间，没有发现任何测试的抗菌药物的耐药分离株比例显著增加。对氨苄西林的耐药性最为常见（14.9%，77/516）。总的来说，有 12 株分离株具有 bla 型 AmpC β-内酰胺酶，7 株分离株产生 CTX-M 型 ESBLs。有一株分离株具有 aac(6')-Ib-cr PMQR 基因。未检测到 qnr 和 qepA 决定子。鉴定出一株属于流行谱系 ST131 的分离株。
我们地区从狗尿液中分离出的大肠杆菌仍然对一线治疗药物敏感，尽管耐药性，特别是对氨苄西林类药物的耐药性，需要监测。这是加拿大首次从伴侣动物中分离出大肠杆菌 ST131。
