中性粒细胞胞外诱捕网是牙周炎与类风湿性关节炎病理生理学之间相互作用的纽带吗？
Are neutrophil extracellular traps the link for the cross-talk between periodontitis and rheumatoid arthritis physiopathology?
作者信息
Oliveira Sicília Rezende, de Arruda José Alcides Almeida, Schneider Ayda Henriques, Carvalho Valessa Florindo, Machado Caio Cavalcante, Corrêa Jôice Dias, Moura Marcela Faria, Duffles Letícia Fernanda, de Souza Flávio Falcão Lima, Ferreira Gilda Aparecida, Costa Fernando Oliveira, Abreu Lucas Guimarães, Taba Júnior Mário, Fukada Sandra Yasuyo, de Oliveira Renê Donizeti Ribeiro, Louzada-Júnior Paulo, Cunha Fernando de Queiroz, Silva Tarcília Aparecida
机构信息
Department of Oral Surgery and Pathology, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Department of Pharmacology, Ribeirão Preto Medical School, Ribeirão Preto, SP, Brazil.
出版信息
Rheumatology (Oxford). 2021 Dec 24;61(1):174-184. doi: 10.1093/rheumatology/keab289.
OBJECTIVES
Neutrophil extracellular traps (NETs) play a role in the pathogenesis of periodontitis and rheumatoid arthritis (RA). However, it remains poorly understood whether NETs participate in the cross-talk between periodontitis and RA. Herein, we investigated the production of NETs in individuals with periodontitis and RA and its association with clinical parameters. The impact of periodontal therapy on RA and NET release was also assessed.
METHODS
The concentration of NETs and cytokines was determined in the saliva and plasma of individuals with early RA (n = 24), established RA (n = 64) and individuals without RA (n = 76). The influence of periodontitis on the production of NETs and cytokines was also evaluated.
RESULTS
Individuals with early RA had a higher concentration of NETs in saliva and plasma than individuals with established RA or without RA. Periodontitis resulted in an increase in the concentration of NETs of groups of individuals without RA and with early RA. The proportion of individuals with high concentrations of IL-6, IL-10 and GM-CSF was higher among individuals with periodontitis than among individuals without periodontitis. The concentrations of TNF-α, IL-6, IL-17/IL-25 and IL-28A were particularly high in individuals with early RA. Worse periodontal clinical parameters, RA onset and RA activity were significantly associated with circulating NETs. Periodontal therapy was associated with a reduction in the concentration of NETs and inflammatory cytokines and amelioration in periodontitis and RA.
CONCLUSION
This study reveals that NETs are a possible link between periodontitis and RA, with periodontal therapy resulting in a dramatic switch in circulating NET levels.
目的
中性粒细胞胞外陷阱（NETs）在牙周炎和类风湿关节炎（RA）的发病机制中起作用。然而，NETs是否参与牙周炎和RA之间的相互作用仍知之甚少。在此，我们研究了牙周炎和RA患者中NETs的产生及其与临床参数的关联。还评估了牙周治疗对RA和NET释放的影响。
方法
测定早期RA患者（n = 24）、确诊RA患者（n = 64）和无RA患者（n = 76）唾液和血浆中NETs及细胞因子的浓度。还评估了牙周炎对NETs和细胞因子产生的影响。
结果
早期RA患者唾液和血浆中NETs的浓度高于确诊RA患者或无RA患者。牙周炎导致无RA组和早期RA组个体的NETs浓度增加。牙周炎患者中IL-6、IL-10和GM-CSF高浓度个体的比例高于无牙周炎患者。早期RA患者中TNF-α、IL-6、IL-17/IL-25和IL-28A的浓度特别高。较差的牙周临床参数、RA发病和RA活动与循环中的NETs显著相关。牙周治疗与NETs浓度和炎性细胞因子的降低以及牙周炎和RA的改善有关。
结论
本研究表明，NETs可能是牙周炎和RA之间的一个联系，牙周治疗导致循环中NET水平发生显著变化。
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