Clinical Laboratory Center, Children's Hospital of Shanxi, Women Health Center of Shanxi, Taiyuan, China.
Hum Fertil (Camb). 2022 Jul;25(3):569-574. doi: 10.1080/14647273.2021.1903099. Epub 2021 Mar 22.
This study aimed to explore the relationship between recurrent reproductive failure (RRF) and blocking antibody (BA) and lymphocytes, to explore the difference of immune status between recurrent spontaneous abortion (RSA) and recurrent implantation failure (RIF) patients. We undertook a retrospective analysis of BA and lymphocyte subsets detected by flow cytometry in 720 RRF patients (411 RSA and 309 RIF patients) who were treated at Shanxi Maternal and Child Health Hospital from April 2015 to October 2019. The BA negative rate of RRF patients was 81.80%, which was significantly higher than that of normal women (23.46%) ( < 0.05). There was no significant difference in BA negative rates between RSA and RIF patients. Compared with the normal value, the percentage of CD3 T lymphocytes and CD3CD4 T lymphocytes in RRF patients were 71.1% (65.9-76.0) and 36.8% (32.5-41.9) respectively, which were significantly lower than the normal value ( < 0.05). The percentage of B cells in RSA patients [11.0 (8.8-13.9)] was significantly lower than that in RIF patients [11.9 (9.4-14.8)]. The occurrence of RRF is related to the lack of BA and the change of lymphoid subsets. There are some differences in immune status between RSA and RIF patients.
本研究旨在探讨反复生殖失败（RRF）与阻断抗体（BA）和淋巴细胞的关系，探索复发性自然流产（RSA）和复发性植入失败（RIF）患者免疫状态的差异。我们对 2015 年 4 月至 2019 年 10 月在山西省妇幼保健院治疗的 720 例 RRF 患者（411 例 RSA 和 309 例 RIF 患者）进行了 BA 和流式细胞术检测的淋巴细胞亚群的回顾性分析。RRF 患者 BA 阴性率为 81.80%，明显高于正常女性（23.46%）（<0.05）。RSA 和 RIF 患者 BA 阴性率无显著差异。与正常值相比，RRF 患者的 CD3 T 淋巴细胞和 CD3CD4 T 淋巴细胞百分比分别为 71.1%（65.9-76.0）和 36.8%（32.5-41.9），明显低于正常值（<0.05）。RSA 患者 B 细胞的百分比[11.0（8.8-13.9）]明显低于 RIF 患者[11.9（9.4-14.8）]。RRF 的发生与 BA 缺乏和淋巴样亚群的改变有关。RSA 和 RIF 患者的免疫状态存在一些差异。
