Fan Junmei, Zhao Jinghui, Zhang Junkun, Chen Yanhua, Bi Xingyu, Liu Huiping, Liu Junfen, Wang Jinbao, Wu Xueqing
Department of Reproductive Medicine Center, Children's Hospital of Shanxi and Women Health Center of Shanxi, Taiyuan, Shanxi, 030013, People's Republic of China.
Department of Pediatric and Adolescent Gynecology, Shenzhen Children's Hospital, Shenzhen, Guangdong, 518000, People's Republic of China.
Patient Prefer Adherence. 2024 Oct 16;18:2125-2134. doi: 10.2147/PPA.S470258. eCollection 2024.
This study aimed to explore the effect of vitamin D supplementation on lymphocyte subsets in infertile women.
The study involved a total of 247 patients who suffered recurrent embryo implantation failure (RIF) or recurrent spontaneous abortion (RSA) between January and December 2019 in the Reproductive Medicine Center of Children's Hospital of Shanxi and Women Health Center of Shanxi. The differences in the vitamin D and lymphocyte subsets of the two diseases, the correlation between the 25-hydroxy vitamin D and lymphocyte subsets, the changes in the lymphocyte subsets after vitamin D supplementation and the impact on pregnancy outcome were analysed.
The prevalence of vitamin D deficiency was 77.33% (191/247). After vitamin D supplementation, there were no significant differences in helper T cells (Th), cytotoxic T cells (Tc), Th/Tc and natural killer cells (NK) ( > 0.05), but there were significant differences in leukocyte differentiation antigens 3+ (CD3+), natural killer T cells (NKT), B lymphocytes and vitamin D ( < 0.05). Before vitamin D supplementation, the difference in the composition ratio of different vitamin D levels between the RIF and RSA groups was not statistically significant. At different vitamin D levels, there were no significant differences in CD3+, Th, Tc, Th/Tc, NK or B lymphocyte ( > 0.05), but there was a significant difference in NKT ( < 0.05).
Vitamin D supplementation can reduce the level of NKT in infertile women, which may be of benefit to the pregnancy outcome of couples who experience RIF.
本研究旨在探讨补充维生素D对不孕女性淋巴细胞亚群的影响。
本研究共纳入247例患者，均于2019年1月至12月在山西省儿童医院生殖医学中心及山西省妇女保健院就诊，患有反复胚胎植入失败（RIF）或反复自然流产（RSA）。分析了两种疾病患者维生素D和淋巴细胞亚群的差异、25-羟基维生素D与淋巴细胞亚群的相关性、补充维生素D后淋巴细胞亚群的变化及其对妊娠结局的影响。
维生素D缺乏的患病率为77.33%（191/247）。补充维生素D后，辅助性T细胞（Th）、细胞毒性T细胞（Tc）、Th/Tc和自然杀伤细胞（NK）无显著差异（>0.05），但白细胞分化抗原3+（CD3+）、自然杀伤T细胞（NKT）、B淋巴细胞和维生素D有显著差异（<0.05）。补充维生素D前，RIF组和RSA组不同维生素D水平的构成比差异无统计学意义。在不同维生素D水平下，CD3+、Th、Tc、Th/Tc、NK或B淋巴细胞无显著差异（>0.05），但NKT有显著差异（<0.05）。
补充维生素D可降低不孕女性NKT水平，这可能对经历RIF的夫妇的妊娠结局有益。
