Department of Cardiology, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders (Chongqing), China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, People's Republic of China.
Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University, Chongqing, 400014, People's Republic of China.
J Nanobiotechnology. 2021 Mar 22;19(1):82. doi: 10.1186/s12951-021-00822-7.
Ischemic heart disease (IHD) is the leading cause of morbidity and mortality worldwide, and imposes a serious economic load. Thus, it is crucial to perform a timely and accurate diagnosis and monitoring in the early stage of myocardial ischemia. Currently, nanoparticles (NPs) have emerged as promising tools for multimodal imaging, because of their advantages of non-invasion, high-safety, and real-time dynamic imaging, providing valuable information for the diagnosis of heart diseases.
In this study, we prepared a targeted nanoprobe (termed IMTP-FeO-PFH NPs) with enhanced ultrasound (US), photoacoustic (PA), and magnetic resonance (MR) performance for direct and non-invasive visual imaging of ischemic myocardium in a rat model. This successfully designed nanoprobe had excellent properties such as nanoscale size, good stability, phase transformation by acoustic droplet vaporization (ADV), and favorable safety profile. Besides, it realized obvious targeting performance toward hypoxia-injured cells as well as model rat hearts. After injection of NPs through the tail vein of model rats, in vivo imaging results showed a significantly enhanced US/PA/MR signal, well indicating the remarkable feasibility of nanoprobe to distinguish the ischemic myocardium.
IMTP-FeO-PFH NPs may be a promising nanoplatform for early detection of ischemic myocardium and targeted treatment under visualization for the future.
缺血性心脏病（IHD）是全球发病率和死亡率的主要原因，并造成了严重的经济负担。因此，在心肌缺血的早期阶段进行及时、准确的诊断和监测至关重要。目前，纳米颗粒（NPs）已成为多模态成像的有前途的工具，因为它们具有非侵入性、高安全性和实时动态成像的优势，为心脏病的诊断提供了有价值的信息。
在这项研究中，我们制备了一种具有增强超声（US）、光声（PA）和磁共振（MR）性能的靶向纳米探针（称为 IMTP-FeO-PFH NPs），用于直接和非侵入性可视化成像大鼠模型中的缺血性心肌。这种成功设计的纳米探针具有纳米级尺寸、良好的稳定性、通过声空化（ADV）的相变、良好的安全性等优良特性。此外，它对缺氧损伤的细胞和模型大鼠心脏实现了明显的靶向性能。通过尾静脉注射 NPs 后，在体成像结果显示 US/PA/MR 信号明显增强，很好地表明了纳米探针在区分缺血性心肌方面的显著可行性。
IMTP-FeO-PFH NPs 可能成为未来可视化下缺血性心肌早期检测和靶向治疗的有前途的纳米平台。
