Shahid Muhammad Furqan, Yaqub Tahir, Ali Muzaffar, Ul-Rahman Aziz, Bente Dennis A
Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Acta Trop. 2021 Jun;218:105892. doi: 10.1016/j.actatropica.2021.105892. Epub 2021 Mar 19.
Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne zoonotic disease of human that caused by CCHF virus. To study the epidemiological distribution of CCHFV, 2183 tick samples were collected from sheep, goats, cattle and buffalo of different livestock farms of ten districts of Punjab province of Pakistan. Detection of CCHFV was done using enzyme link immunosorbent assay (ELISA) after proper identification of tick samples. The partial S-segment of CCHFV from ELISA positive tick samples was amplified by PCR and sequenced to determine the genotype of CCHFV. Out of2183 collected tick samples, 1913 ticks belonged to 5 species of genus Hyalomma as H. antolicum (48%), H. marginatum (30.2%), H. rufipes (10.82%), H. impressum (5.43%) and H. dromedarii (5.27%). While 270 ticks belonged to 3 species of genus Rhipicephalus as R. microplus (44.8%), R. sanguineus (32.22%) and R. turanicus (24.8%). The overall antigenic prevalence of CCHFV was found to be 12.13% in collected tick samples and 21 tick pools were sequenced for partial S-segment of CCHFV. All of the 21 tick pools were clustered in genotype IV (Asia-1). The highest prevalence of CCHFV was found in district Chakwal (24.13%) followed by Mianwali (23.68%), Rawalpindi (23.07%), Attock (20.0%), Rajanpur (10.52%) and Lahore (8.33%). In positive tick pools, the highest prevalence of CCHFV antigen was found in H. antolicum (39.6%) followed by H. marginatum (30.18%), H. rufipes (13.2%), H. impressum (3.77%), H. dromedarii (1.88%), R. microplus (5.66%) and R. sanguineus (5.66%). The current study confirms the presence of CCHFV in the ticks population of Punjab. The CCHF virus present in Punjab belongs to Asia-1 genotype. It is important to control the tick infestation of the animals present in these areas. So that the transmission cycle of CCHF can be inhibited.
克里米亚-刚果出血热（CCHF）是一种由CCHF病毒引起的经蜱传播的人畜共患病。为研究CCHFV的流行病学分布，从巴基斯坦旁遮普省10个区不同养殖场的绵羊、山羊、牛和水牛身上采集了2183份蜱样本。在对蜱样本进行正确鉴定后，使用酶联免疫吸附测定（ELISA）检测CCHFV。通过PCR扩增ELISA阳性蜱样本中CCHFV的部分S片段并测序，以确定CCHFV的基因型。在采集的2183份蜱样本中，1913只蜱属于璃眼蜱属的5个物种，即安氏璃眼蜱（48%）、边缘璃眼蜱（30.2%）、红足璃眼蜱（10.82%）、印记璃眼蜱（5.43%）和单峰驼璃眼蜱（5.27%）。而270只蜱属于扇头蜱属的3个物种，即微小扇头蜱（44.8%）、血红扇头蜱（32.22%）和图兰扇头蜱（24.8%）。在所采集的蜱样本中，CCHFV的总体抗原阳性率为12.13%，对21个蜱样本池进行了CCHFV部分S片段的测序。所有21个蜱样本池均聚类为IV型基因型（亚洲-1型）。CCHFV阳性率最高的是查克拉尔区（24.13%），其次是米亚瓦利（23.68%）、拉瓦尔品第（23.07%）、阿托克（20.0%）、拉詹布尔（10.52%）和拉合尔（8.33%）。在阳性蜱样本池中，CCHFV抗原阳性率最高的是安氏璃眼蜱（39.6%），其次是边缘璃眼蜱（30.18%）、红足璃眼蜱（13.2%）、印记璃眼蜱（3.77%）、单峰驼璃眼蜱（1.88%）、微小扇头蜱（5.66%）和血红扇头蜱（5.66%）。本研究证实旁遮普省蜱虫种群中存在CCHFV。旁遮普省存在的CCHF病毒属于亚洲-1型基因型。控制这些地区动物的蜱虫感染很重要。这样可以抑制CCHF的传播循环。
