一种同属筛选方法揭示了新型效应子以及研究发病机制的新可能性。
A Same-Genus Screening Approach Reveals Novel Effectors and New Possibilities for Investigating Pathogenesis.
作者信息
Ryan John D, Nelson David E
机构信息
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, USA.
出版信息
J Bacteriol. 2021 Jun 1;203(11). doi: 10.1128/JB.00157-21. Epub 2021 Mar 22.
Abstract
are obligate intracellular pathogens that rely on secreted effector proteins to establish their intracellular niche. In this issue of the , Yanatori describe a screen for effectors, performed in , which identified several new proteins that are translocated during infection (Yanatori, Miura et al. 2021). More broadly, they demonstrate how new genetic approaches in can be used to characterize the virulence factors of other species.
摘要
是专性细胞内病原体，依赖分泌的效应蛋白来建立其细胞内生态位。在本期《 》中，柳取等描述了在 中进行的针对 效应蛋白的筛选，该筛选鉴定出了几种在感染过程中易位的新蛋白（柳取、三浦等，2021年）。更广泛地说，他们展示了 中的新遗传方法如何可用于表征其他 物种的毒力因子。
相似文献
1
A Same-Genus Screening Approach Reveals Novel Effectors and New Possibilities for Investigating Pathogenesis.
J Bacteriol. 2021 Jun 1;203(11). doi: 10.1128/JB.00157-21. Epub 2021 Mar 22.
2
Application of a expression system to identify proteins translocated into host cells.
J Bacteriol. 2021 Jun 1;203(11). doi: 10.1128/JB.00511-20. Epub 2021 Mar 8.
3
Characterization of a secreted Chlamydia protease.
Cell Microbiol. 2002 Jul;4(7):411-24. doi: 10.1046/j.1462-5822.2002.00200.x.
4
Impact of Active Metabolism on Chlamydia trachomatis Elementary Body Transcript Profile and Infectivity.
J Bacteriol. 2018 Jun 25;200(14). doi: 10.1128/JB.00065-18. Print 2018 Jul 15.
5
Targeted Disruption of Chlamydia trachomatis Invasion by in Trans Expression of Dominant Negative Tarp Effectors.
Front Cell Infect Microbiol. 2016 Aug 23;6:84. doi: 10.3389/fcimb.2016.00084. eCollection 2016.
6
A secondary structure motif predictive of protein localization to the chlamydial inclusion membrane.
Cell Microbiol. 2000 Feb;2(1):35-47. doi: 10.1046/j.1462-5822.2000.00029.x.
7
A directed screen for chlamydial proteins secreted by a type III mechanism identifies a translocated protein and numerous other new candidates.
Mol Microbiol. 2005 Jun;56(6):1636-47. doi: 10.1111/j.1365-2958.2005.04647.x.
8
The Type III Secretion Effector CteG Mediates Host Cell Lytic Exit of .
Front Cell Infect Microbiol. 2022 Jul 8;12:902210. doi: 10.3389/fcimb.2022.902210. eCollection 2022.
9
A meta-analysis of affinity purification-mass spectrometry experimental systems used to identify eukaryotic and chlamydial proteins at the Chlamydia trachomatis inclusion membrane.
J Proteomics. 2020 Feb 10;212:103595. doi: 10.1016/j.jprot.2019.103595. Epub 2019 Nov 21.
10
Multiple Chlamydiaceae species in trachoma: implications for disease pathogenesis and control.
PLoS Med. 2008 Jan 3;5(1):e14. doi: 10.1371/journal.pmed.0050014.
引用本文的文献
1
A Chlamydial Plasmid-Dependent Secretion System for the Delivery of Virulence Factors to the Host Cytosol.
mBio. 2021 Jun 29;12(3):e0117921. doi: 10.1128/mBio.01179-21. Epub 2021 Jun 8.
本文引用的文献
1
Application of a expression system to identify proteins translocated into host cells.
J Bacteriol. 2021 Jun 1;203(11). doi: 10.1128/JB.00511-20. Epub 2021 Mar 8.
2
Comparative Genome Analysis of 33 Strains Reveals Characteristic Features of and Closely Related Species.
Pathogens. 2020 Oct 28;9(11):899. doi: 10.3390/pathogens9110899.
3
Development of a Plasmid Shuttle Vector System for Genetic Manipulation of Chlamydia psittaci.
mSphere. 2020 Aug 26;5(4):e00787-20. doi: 10.1128/mSphere.00787-20.
4
The multiple functions of the numerous secreted proteins: the tip of the iceberg.
Microb Cell. 2019 Aug 21;6(9):414-449. doi: 10.15698/mic2019.09.691.
5
: Diseases in Primary Hosts and Zoonosis.
Microorganisms. 2019 May 24;7(5):146. doi: 10.3390/microorganisms7050146.
6
The Genetic Transformation of Chlamydia pneumoniae.
mSphere. 2018 Oct 10;3(5):e00412-18. doi: 10.1128/mSphere.00412-18.
7
Assembly, structure, function and regulation of type III secretion systems.
Nat Rev Microbiol. 2017 Jun;15(6):323-337. doi: 10.1038/nrmicro.2017.20. Epub 2017 Apr 10.
8
Chlamydia cell biology and pathogenesis.
Nat Rev Microbiol. 2016 Jun;14(6):385-400. doi: 10.1038/nrmicro.2016.30. Epub 2016 Apr 25.
9
Identification of type III secretion substrates of Chlamydia trachomatis using Yersinia enterocolitica as a heterologous system.
BMC Microbiol. 2014 Feb 17;14:40. doi: 10.1186/1471-2180-14-40.
10
Genus-optimized strategy for the identification of chlamydial type III secretion substrates.
Pathog Dis. 2013 Dec;69(3):213-22. doi: 10.1111/2049-632X.12070. Epub 2013 Aug 14.
Zotero 插件