Department of Oral and Maxillofacial Prosthodontics, King Abdulaziz University, Faculty of Dentistry, Jeddah, Saudi Arabia; Eastman Dental Institute, University College London, Oral Pathology Department, London, United Kingdom.
Department of Oral Biology, King Abdulaziz University, Faculty of Dentistry, Jeddah, Saudi Arabia.
Indian J Cancer. 2022 Jan-Mar;59(1):65-72. doi: 10.4103/ijc.IJC_599_19.
Human papillomavirus (HPV) is an evolving important risk factor for head and neck cancer (HNC), especially for individuals who do not smoke and drink alcohol. The aim of this study was to establish the prevalence of HPV infection and elucidate its association with head and neck squamous cell carcinoma (HNSCC) patients in UK population.
The presence and association of HPV was investigated in HNSCC patients in this retrospective clinical study. Samples were obtained from archived biopsies and resections. HPV screening was performed by the use of polymerase chain reaction (PCR) using the GP5/GP6 and the SPF1/2 consensus as primers and by immunohistochemistry (IHC). Samples of viral warts that were IHC positive for HPV and fibroepethelial polyps (FEP) were used, as positive and negative controls, respectively.
The cohort included 124 patients with HNSCC with an age range of 27-97 years (median, 60 years) and a male to female ratio of 2:1. Among the 124 HNSCC, 43/124 (34.7%) were from the tongue, 74/124 (60%) presented with advanced stage III or IV disease, 112/124 (90%) had a conventional phenotype, 84/124 (68%) were moderately differentiated, and 89/124 (72%) had bands or cords at the invasive front. Of the 124 patients with HNSCC, 84/124 (68%) demonstrated the presence of HPV, 0/124 (0%) was for oral squamous cell carcinomas (OSCC). HPV16 was the associated virus type in all positive samples. However, no significant association was observed between HPV positivity and other clinico-pathological variables including age and gender of the patients, stage, and malignancy differentiation.
The results we provide suggest that HPV infection is low in HNSCC, in general, and absent in OSCC, specifically, in this UK population during this time period. This implies that HPV infection may not play an important role in HNSCC carcinogenesis compared to other risk factors in UK population. This information can aid in more effective treatment approaches for treating UK cases of HNSCC.
人乳头瘤病毒（HPV）是头颈部癌症（HNC）的一个重要的不断演变的风险因素，尤其是对那些不吸烟和饮酒的人。本研究的目的是确定 HPV 感染在英国人群中的流行情况，并阐明其与头颈部鳞状细胞癌（HNSCC）患者的关系。
在这项回顾性临床研究中，我们调查了 HNSCC 患者中 HPV 的存在和相关性。从存档的活检和切除标本中获取样本。使用聚合酶链反应（PCR），使用 GP5/GP6 和 SPF1/2 共识作为引物，以及免疫组织化学（IHC）检测 HPV 的存在。使用 HPV 免疫组织化学阳性的病毒性疣和纤维上皮息肉（FEP）样本作为阳性和阴性对照。
该队列包括 124 名 HNSCC 患者，年龄在 27-97 岁之间（中位数为 60 岁），男女比例为 2:1。在 124 例 HNSCC 中，43/124（34.7%）来自于舌，74/124（60%）处于晚期 III 或 IV 期，112/124（90%）具有常规表型，84/124（68%）为中度分化，89/124（72%）在侵袭前沿具有带状或条索状。在 124 例 HNSCC 患者中，84/124（68%）显示 HPV 存在，0/124（0%）为口腔鳞状细胞癌（OSCC）。所有阳性样本均为 HPV16 相关病毒类型。然而，HPV 阳性与包括患者年龄和性别、分期和恶性程度分化在内的其他临床病理变量之间没有显著相关性。
我们提供的结果表明，HPV 感染在 HNSCC 中总体较低，特别是在这段时间内的英国人群中不存在 OSCC。这意味着与英国人群中的其他风险因素相比，HPV 感染在 HNSCC 的致癌作用中可能并不重要。这些信息可以帮助为英国的 HNSCC 病例提供更有效的治疗方法。
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