Department of Periodontology, College of Dentistry, Hawler Medical University, Erbil, Iraq.
Department of Pathology, College of Medicine, Hawler Medical University, Erbil, Iraq.
Indian J Dent Res. 2020 Nov-Dec;31(6):893-898. doi: 10.4103/ijdr.IJDR_909_19.
The present study aimed to investigate the efficacy of local use of propolis (PRO) as a monotherapy or adjunctive therapy to scaling and root planning (SRP) in the treatment of ligature-induced periodontitis (LIP) in rats by evaluating inflammatory markers and oxidative stress.
The present study was carried out at Hawler Medical University, College of Dentistry, and the animals used in the study were rats that were housed in an animal facility at the College of Medicine. The experimental part of the study was carried between December 2016 and August 2017.
In total, 125 rats were equally divided into 5 groups of 25 animals: the control group (C) with no experimental periodontitis or treatment (gp1), LIP treated by local irrigation with a vehicle (dimethyl sulfoxide + physiological saline solution) group (gp2); LIP treated by SRP with vehicle irrigation group (gp3); LIP treated by local irrigation with 10% PRO extract group (gp4); and LIP treated by SRP with 10% PRO extract irrigation group (gp5). Experimental periodontitis was induced by placing a silk ligature around the cervix of the mandibular incisor for 4 weeks. After removal of the ligature, SRP was performed in gp3 and gp5. Five rats from each group were euthanized on days 0, 3, 7, 14, and 21 after local treatment. Intracardiac blood samples were obtained for analysis of the inflammatory cytokines interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) and oxidative stress, which were represented by the expression of serum malondialdehyde (MDA).
Data were analyzed statistically by analysis of variance followed by Tukey's test (P < 0.05).
Gp5 demonstrated significantly lower IL-1β, TNF-α, and MDA serum levels than the other treatment groups at all experimental time points (P < 0.05). No significant differences were found when PRO was used as a monotherapy (P > 0.05).
Local use of a PRO extract was effective as an adjunctive treatment with SRP in a rat model of experimental periodontitis via anti-inflammatory and antioxidative activities.
本研究旨在通过评估炎症标志物和氧化应激，探讨局部使用蜂胶（PRO）作为单独疗法或辅助治疗在治疗大鼠结扎诱导性牙周炎（LIP）中的疗效。
本研究在库尔德斯坦医科大学牙科学院进行，研究中使用的动物为在医学院动物设施中饲养的大鼠。研究的实验部分于 2016 年 12 月至 2017 年 8 月进行。
共有 125 只大鼠等分为 5 组，每组 25 只：无实验性牙周炎或治疗的对照组（C）（gp1）；用载剂（二甲亚砜+生理盐水）局部冲洗治疗的 LIP 组（gp2）；用载剂冲洗行牙周洁治术（SRP）治疗的 LIP 组（gp3）；用 10% PRO 提取物局部冲洗治疗的 LIP 组（gp4）；用 10% PRO 提取物冲洗行 SRP 治疗的 LIP 组（gp5）。通过在下颌切牙颈周围放置丝线结扎物 4 周诱导实验性牙周炎。结扎物去除后，在 gp3 和 gp5 中进行 SRP。每组各有 5 只大鼠在局部治疗后第 0、3、7、14 和 21 天处死。通过分析白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）等炎症细胞因子和丙二醛（MDA）等氧化应激来检测血清中的表达。
采用方差分析，然后进行 Tukey 检验（P < 0.05）进行统计分析。
gp5 在所有实验时间点的血清 IL-1β、TNF-α和 MDA 水平均显著低于其他治疗组（P < 0.05）。当 PRO 作为单独疗法使用时，没有发现显著差异（P > 0.05）。
在大鼠实验性牙周炎模型中，局部使用 PRO 提取物作为牙周洁治术的辅助治疗具有抗炎和抗氧化作用。
