Group of Research and Study on Laser in Dentistry (GEPLO), Division of Periodontics, Department of Surgery and Integrated Clinic, University Estadual Paulista (UNESP), Araçatuba, Brazil.
Master Course, Barretos Dental School, University Center of the Educational Foundation of Barretos (UNIFEB), Barretos, Brazil.
J Periodontal Res. 2016 Feb;51(1):26-37. doi: 10.1111/jre.12274. Epub 2015 Apr 27.
This study assessed the effects of the local use of Saccharomyces cerevisiae as monotherapy and as an adjuvant to the mechanical treatment of ligature-induced periodontitis in rats.
Periodontitis was induced in 72 rats via the installation of a ligature around the mandibular first molar. After 7 d, the ligature was removed and the rats were placed in one of the following groups: no treatment (C; n = 18); scaling and root planing (SRP; n = 18); local irrigation with probiotics (PRO; n = 18); and SRP followed by local irrigation with probiotics (SRP/PRO; n = 18). Six rats from each group were killed at 7, 15 and 30 d. The histological characteristics, alveolar bone loss (ABL) and immunolabeling of tumor necrosis factor alpha (TNF-α), interleukin-1beta (IL-1β), interleukin-10 (IL-10) and TRAP on the furcation area of the first molar were assessed.
The PRO group showed features of acceleration of the tissue-repair process during the entire experiment. On day 15, there was less ABL in the SRP/PRO group compared with the C group. There were fewer TRAP-positive cells in the SRP and SRP/PRO groups at 30 d. There was less immunostaining for TNF-α in the PRO and SRP/PRO groups and less immunostaining for IL-1β in the PRO group. However, there was more immunostaining for IL-10 in the PRO group on day 15.
Local use of the probiotic did not result in any adverse effects on periodontal tissues. When used as monotherapy or as an adjuvant, the probiotic was effective at controlling periodontitis in rats.
本研究评估了局部使用酿酒酵母作为单一疗法以及作为机械治疗结扎诱导的大鼠牙周炎的辅助治疗的效果。
通过在下颌第一磨牙周围安装结扎线诱导 72 只大鼠发生牙周炎。7d 后，去除结扎线，并将大鼠分为以下几组：不治疗（C；n = 18）；刮治和根面平整（SRP；n = 18）；局部灌洗益生菌（PRO；n = 18）；SRP 后局部灌洗益生菌（SRP/PRO；n = 18）。每组各有 6 只大鼠在第 7、15 和 30 天处死。评估第一磨牙叉区的组织学特征、牙槽骨丧失（ABL）和肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-10（IL-10）和破骨细胞（TRAP）的免疫标记。
PRO 组在整个实验过程中表现出加速组织修复过程的特征。第 15 天，SRP/PRO 组的 ABL 比 C 组少。第 30 天，SRP 和 SRP/PRO 组中的 TRAP 阳性细胞较少。PRO 和 SRP/PRO 组中的 TNF-α免疫染色较少，PRO 组中的 IL-1β免疫染色较少。然而，PRO 组在第 15 天的 IL-10 免疫染色较多。
局部使用益生菌对牙周组织没有任何不良影响。作为单一疗法或辅助治疗，益生菌对控制大鼠牙周炎有效。
