Jin Yang, Harvey Thomas Nelson, Bartosova Zdenka, Hassani Sahar, Bruheim Per, Sandve Simen Rød, Vik Jon Olav
Center of Integrative Genetics (CIGENE), Norwegian University of Life Sciences, 1430 Aas, Norway.
Department of Biotechnology and Food Science, Norwegian University of Science and Technology, 7491 Trondheim, Norway.
J Agric Food Chem. 2021 Mar 31;69(12):3787-3796. doi: 10.1021/acs.jafc.0c07281. Epub 2021 Mar 23.
Salmon is an important source of long-chain highly unsaturated fatty acids (LC-HUFAs) such as 22:6-3 [docosahexaenoic acid (DHA)]. In the present study, we conducted two identical experiments on salmon in freshwater (FW) and seawater (SW) stages, with a diet switch from fish oil (high in LC-HUFA) to vegetable oil (low in LC-HUFA) and vice versa. Our aim was to investigate the diet and life stage-specific features of lipid uptake (gut), processing (liver), and deposition (muscle). The lipid composition changed much faster in the gut of SW fish relative to FW fish, suggesting that the former had a higher rate of lipid absorption and transport. SW fish also had higher expression of phospholipid synthesis and lipoprotein formation genes in the gut, whereas FW fish had higher expression of lipid synthesis genes in the liver. All phospholipids except PC-44:12 and PE-44:12 were less abundant in SW, suggesting that SW fish have a higher requirement for DHA.
三文鱼是长链高度不饱和脂肪酸（LC-HUFAs）的重要来源，如22:6-3[二十二碳六烯酸（DHA）]。在本研究中，我们对处于淡水（FW）和海水（SW）阶段的三文鱼进行了两项相同的实验，饮食从富含LC-HUFA的鱼油转换为低LC-HUFA的植物油，反之亦然。我们的目的是研究脂质摄取（肠道）、加工（肝脏）和沉积（肌肉）在饮食和生活阶段的特异性特征。相对于FW鱼，SW鱼肠道中的脂质组成变化更快，这表明前者具有更高的脂质吸收和运输速率。SW鱼肠道中磷脂合成和脂蛋白形成基因的表达也更高，而FW鱼肝脏中脂质合成基因的表达更高。除了PC-44:12和PE-44:12之外，所有磷脂在SW中的含量都较低，这表明SW鱼对DHA的需求量更高。
