Jephcott E H, McMillen I C, Congdon P, Thorburn G D
Department of Physiology, Monash University, Clayton, Victoria, Australia.
Res Vet Sci. 1988 Jan;44(1):21-4.
Whether the increases in plasma cortisol concentrations which are associated with electroimmobilization (E-IM) of conscious sheep are modified by current intensity, current duration or prior treatment with diazepam was assessed. Sheep were electroimmobilized at 30, 40 or 60 mA for one minute and at 40 mA for two or four minutes. After E-IM of one minute's duration, peak plasma cortisol concentrations occurred at 20 minutes and were 39.1 +/- 7.8 ng ml-1 at 0 mA (control), 56.7 +/- 9.6 ng ml-1 at 30 mA, 62.1 +/- 6.9 ng ml-1 at 40 mA and 78.4 +/- 3.2 ng ml-1 at 60 mA. Both the peak plasma cortisol concentrations and the integrated cortisol responses to E-Im were correlated with current intensity (P less than 0.01). The cortisol responses to E-Im at 40 mA for one, two and four minutes were not significantly different and the response to E-IM at 40 mA for one minute was not affected by the administration of diazepam (0.2 mg kg-1 intravenously) 15 minutes before E-IM. These results indicate that the cortisol response to E-IM is directly dependent on current intensity and that adrenocortical activation by E-IM may be minimised by choosing the lowest current at which effective restraint is maintained.
研究评估了与清醒绵羊电固定（E-IM）相关的血浆皮质醇浓度升高是否会因电流强度、电流持续时间或地西泮预处理而改变。绵羊分别以30、40或60毫安的电流电固定1分钟，或以40毫安的电流电固定2或4分钟。在持续1分钟的E-IM后，血浆皮质醇浓度峰值在20分钟出现，0毫安（对照）时为39.1±7.8纳克/毫升，30毫安时为56.7±9.6纳克/毫升，40毫安时为62.1±6.9纳克/毫升，60毫安时为78.4±3.2纳克/毫升。血浆皮质醇浓度峰值和对E-Im的皮质醇综合反应均与电流强度相关（P<0.01）。40毫安下电固定1、2和4分钟时的皮质醇反应无显著差异，且在E-IM前15分钟静脉注射地西泮（0.2毫克/千克）不影响40毫安下电固定1分钟时的反应。这些结果表明，对E-IM的皮质醇反应直接取决于电流强度，并且通过选择能维持有效约束的最低电流，可将E-IM引起的肾上腺皮质激活降至最低。
