Punniyakotti Parthipan, Aruliah Rajasekar, Angaiah Subramania
Electro-Materials Research Laboratory, Centre for Nanoscience and Technology, Pondicherry University, Puducherry, 605014 India.
Environmental Molecular Microbiology Research Laboratory, Department of Biotechnology, Thiruvalluvar University, Vellore, 632115 India.
3 Biotech. 2021 Apr;11(4):157. doi: 10.1007/s13205-021-02689-9. Epub 2021 Mar 5.
In the present study, an eco-friendly approach is adapted for the synthesis of reduced graphene oxide (rGO's) by a simple hydrothermal reaction using two plant extracts namely and . After the hydrothermal reaction, GO turns into a black color from brown color, which indicates the successful reduction of graphene oxide. Further, various characterization techniques such as UV-Vis spectroscopy, Raman spectroscopy, Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction is used to confirm the physicochemical properties of synthesized rGO's. Raman analysis confirms the reduction of GO by noticing an increase in the I/I ratio significantly. Field emission scanning electron microscopy and transmission electron microscopy clearly show the morphology and crystalline nature of rGO's. FT-IR spectrum confirms that the bioactive molecules of the plant extract (i.e. polyphenols, flavonoids, terpenoids, etc.) playing a key role in the elimination of oxygen groups from the GO surface. Further, the synthesized rGO's are tested for their potential against human lung and breast cancer cell lines. A significant cancer cell inhibition activity is obtained even in the less concentration of rGO's with IC values for lung cancer cell lines are 38.46 µg/mL and 26.69 µg/mL for AIrGO and RSrGO, respectively. Similarly, IC values for breast cancer cell lines are 35.97 µg/mL and 33.22 µg/mL for AIrGO and RSrGO, respectively.
在本研究中，采用一种环保方法，通过简单的水热反应，使用两种植物提取物合成还原氧化石墨烯（rGO）。水热反应后，氧化石墨烯（GO）从棕色变为黑色，这表明氧化石墨烯成功还原。此外，使用紫外可见光谱、拉曼光谱、傅里叶变换红外光谱（FT-IR）和X射线衍射等各种表征技术来确认合成的rGO的物理化学性质。拉曼分析通过显著观察到I/I比的增加来确认GO的还原。场发射扫描电子显微镜和透射电子显微镜清楚地显示了rGO的形态和晶体性质。FT-IR光谱证实植物提取物的生物活性分子（即多酚、黄酮类化合物、萜类化合物等）在从GO表面消除氧基团方面起关键作用。此外，测试了合成的rGO对人肺癌和乳腺癌细胞系的潜在作用。即使在较低浓度的rGO下也获得了显著的癌细胞抑制活性，对于肺癌细胞系，AIrGO和RSrGO的IC值分别为38.46 μg/mL和26.69 μg/mL。同样，对于乳腺癌细胞系，AIrGO和RSrGO的IC值分别为35.97 μg/mL和33.22 μg/mL。
