利用具有聚集诱导发射特性的“开启”荧光探针检测尿白蛋白。

Detection of Urinary Albumin Using a "Turn-on" Fluorescent Probe with Aggregation-Induced Emission Characteristics.

机构信息

Medical Device Research Institute, College of Science and Engineering, Flinders University, South Australia, 5042, Australia.

Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Victoria, 3086, Australia.

出版信息

Chem Asian J. 2021 May 17;16(10):1245-1252. doi: 10.1002/asia.202100180. Epub 2021 Apr 1.

DOI:10.1002/asia.202100180

PMID:33759376

Abstract

Human serum albumin (HSA) is a broadly used biomarker for the diagnosis of various diseases such as chronic kidney disease. Here, a fluorescent probe TC426 with aggregation-induced emission (AIE) characteristics is reported as a sensitive and specific probe for HSA. This probe is non-emissive in aqueous solution, meanwhile it shows bright fluorescence upon interacting with HSA, which makes it applicable in detecting HSA with a high signal to noise ratio. Besides, the fluorescence of TC426 exhibits a high linear correlation with the concentration of albumin in the range of microalbumin (20-200 mg/L), which has a significant importance for the early diagnosis of glomerulus related diseases. Compared with previously reported HSA probes TPE-4TA and BSPOTPE, TC426 shows comparable anti-interference ability towards creatinine and other major components in urine but is excited by a longer excitation wavelength at the visible light range. Finally, with the established assay, TC426 shows excellent performance in detecting HSA in real human urine, indicating its great potential in practical urinalysis.

摘要

人血清白蛋白（HSA）是一种广泛用于诊断各种疾病的生物标志物，如慢性肾病。在这里，我们报道了一种具有聚集诱导发光（AIE）特性的荧光探针 TC426，它是一种用于 HSA 的灵敏和特异的探针。该探针在水溶液中不发光，而在与 HSA 相互作用时显示出明亮的荧光，这使其适用于具有高信噪比的 HSA 检测。此外，TC426 的荧光强度与白蛋白的浓度在微白蛋白（20-200mg/L）范围内呈高度线性相关，这对肾小球相关疾病的早期诊断具有重要意义。与先前报道的 HSA 探针 TPE-4TA 和 BSPOTPE 相比，TC426 对肌酐和尿液中其他主要成分的抗干扰能力相当，但在可见光范围内的激发波长更长。最后，在建立的测定方法中，TC426 在检测真实人尿液中的 HSA 时表现出优异的性能，表明其在实际尿液分析中具有巨大的潜力。