State Key Laboratory of Metastable Materials Science and Technology, Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, P. R. China.
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.
Small. 2021 May;17(19):e2008114. doi: 10.1002/smll.202008114. Epub 2021 Mar 24.
Nanoassemblies based on self-assembly of biological building blocks are promising in mimicking the nanostructures, properties, and functionalities of natural enzymes. However, it remains a challenge to design of biomimetic nanozymes with tunable nanostructures and enhanced catalytic activities starting from simple biomolecules. Herein, the construction of nanoassemblies through coassembly of an amphiphilic amino acid and hemin is reported. The nanostructures and morphologies of the resulting nanoassemblies are readily controlled by tuning the molar ratio between the amino acid and hemin, thus leading to tailored peroxidase-mimicking activities of the nanoassemblies. Importantly, the optimized nanoassemblies exhibit a remarkable catalytic efficiency that is comparable to the natural counterpart when considering molecular mass along with good robustness in multiple catalytic cycles. The nanoassemblies are effectively integrated as biomimetic nanozymes in a sensing system for catalytic detection of glucose. Therefore, this work demonstrates that nanozymes with advanced catalytic capabilities can be constructed by self-assembly of minimalist biological building blocks and may thus promote the rational design and catalytic applications of biomimetic nanozymes.
基于生物构建基元自组装的纳米组装体在模拟天然酶的纳米结构、性质和功能方面具有广阔的前景。然而，从简单的生物分子出发，设计具有可调纳米结构和增强催化活性的仿生纳米酶仍然是一个挑战。本文报道了通过两亲性氨基酸和血红素共组装构建纳米组装体。通过调节氨基酸和血红素之间的摩尔比，很容易控制所得纳米组装体的纳米结构和形态，从而实现纳米组装体过氧化物酶模拟活性的定制。重要的是，优化后的纳米组装体在考虑分子质量的情况下表现出与天然对应物相当的显著催化效率，并且在多个催化循环中具有良好的稳定性。纳米组装体作为仿生纳米酶有效地集成在一个传感系统中，用于催化检测葡萄糖。因此，这项工作表明，具有先进催化能力的纳米酶可以通过最小化的生物构建基元的自组装来构建，从而可能促进仿生纳米酶的合理设计和催化应用。
