Yang Lei, Xing Binglin, Li Fen, Wang Li Kui, Yuan Linlin, Mbuji Amosi Leonard, Peng Zhengqiang, Malhat Farag, Wu Shaoying
Hainan University, Haikou, Hainan, China.
Department of Resources Utilization and Plant Protection, College of Resources and Environmental Science, China Agricultural University, Beijing, Beijing, China.
PeerJ. 2021 Aug 24;9:e12069. doi: 10.7717/peerj.12069. eCollection 2021.
(J. E. Smith), commonly known as fall armyworm (FAW), is one of the most destructive agricultural pests in the world and has posed a great threat to crops. The improper use of insecticides has led to rapid development of resistance. However, the genetic data available for uncovering the insecticide resistance mechanisms are scarce.
In this study, we used PacBio single-molecule real-time (SMRT) sequencing aimed at revealing the full-length transcriptome profiling of the FAW larval brain to obtain detoxification genes.
A total of 18,642 high-quality transcripts were obtained with an average length of 2,371 bp, and 11,230 of which were successfully annotated in six public databases. Among these, 5,692 alternative splicing events were identified.
草地贪夜蛾（J. E. Smith），通常被称为秋粘虫（FAW），是世界上最具破坏性的农业害虫之一，对农作物构成了巨大威胁。杀虫剂的不当使用导致了抗性的快速发展。然而，可用于揭示抗药性机制的遗传数据却很稀少。
在本研究中，我们使用PacBio单分子实时（SMRT）测序技术，旨在揭示草地贪夜蛾幼虫大脑的全长转录组图谱，以获得解毒基因。
共获得18,642条高质量转录本，平均长度为2,371 bp，其中11,230条在六个公共数据库中成功注释。其中，鉴定出5,692个可变剪接事件。
