Abe Y, Okamura K, Hamazaki Y, Wada Y, Yajima A
Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai.
Tohoku J Exp Med. 1988 Mar;154(3):245-51. doi: 10.1620/tjem.154.245.
Monoclonal antibodies have been raised against cytotrophoblast. Two different antigens, defined on cytotrophoblast but not on syncytiotrophoblast were designated ACT-1 and ACT-2, respectively. Chorionic villi were taken from normal early pregnancy and processed for immunization by two different procedures. ACT-1 was demonstrated to be present in lung alveolar cells, endothelial mucosa of the jejunum, colon, ureter, urinary bladder and the fallopian tube, and endometrial gland of the pregnant uterus. On the other hand, ACT-2 was present in the endothelial mucosa of the stomach, endothelium of the renal vessel, and the decidua of the pregnant uterus. Although the monoclonal antibodies did not react with such established cell lines as Bewo, SCH, OVK-18, HHUA, MK-01, FL, BHK and P3 X 63Ag 8 . 653, they did react with some of the cell lines when the cell membrane was destroyed with Triton X-100. Each antibody, therefore, may recognize the antigen not on the cell membrane but in the cytoplasm. The antigens might be shed or may disappear in the process of differentiation from cytotrophoblast to syncytiotrophoblast.
已制备出针对细胞滋养层的单克隆抗体。在细胞滋养层上鉴定出但在合体滋养层上未鉴定出的两种不同抗原，分别命名为ACT - 1和ACT - 2。取自正常早孕的绒毛膜绒毛，通过两种不同的程序进行免疫处理。结果表明，ACT - 1存在于肺泡细胞、空肠、结肠、输尿管、膀胱和输卵管的内皮黏膜以及妊娠子宫的子宫内膜腺中。另一方面，ACT - 2存在于胃的内皮黏膜、肾血管内皮和妊娠子宫的蜕膜中。尽管这些单克隆抗体不与诸如Bewo、SCH、OVK - 18、HHUA、MK - 01、FL、BHK和P3 X 63Ag 8.653等已建立的细胞系发生反应，但当用Triton X - 100破坏细胞膜时，它们确实与其中一些细胞系发生反应。因此，每种抗体可能识别的抗原不在细胞膜上，而是在细胞质中。这些抗原可能在从细胞滋养层向合体滋养层分化的过程中脱落或消失。
