Sadat Md Abu, Ullah Md Wali, Bashar Kazi Khayrul, Hossen Quazi Md Mosaddeque, Tareq Md Zablul, Islam Md Shahidul
Basic and Applied Research on Jute Project, Bangladesh Jute Research Institute, Manik Mia Avenue, Dhaka, 1207, Bangladesh.
J Genet Eng Biotechnol. 2021 Mar 24;19(1):46. doi: 10.1186/s43141-021-00143-0.
In fungi, like other eukaryotes, protein turnover is an important cellular process for the controlling of various cellular functions. The ubiquitin-proteasome pathway degrades some selected intracellular proteins and F-box proteins are one of the important components controlling protein degradation. F-box proteins are well studied in different model plants however, their functions in the fungi are not clear yet. This study aimed to identify the genes involved in protein degradation for disease development in the Macrophomina phaseolina fungus.
In this research, in silico studies were done to understand the distribution of F-box proteins in pathogenic fungi including Macrophomina phaseolina fungus. Genome-wide analysis indicates that M. phaseolina fungus contained thirty-one F-box proteins throughout its chromosomes. In addition, there are 17, 37, 16, and 21 F-box proteins have been identified from Puccinia graminis, Colletotrichum graminicola, Ustilago maydis, and Phytophthora infestans, respectively. Analyses revealed that selective fungal genomes contain several additional functional domains along with F-box domain. Sequence alignment showed the substitution of amino acid in several F-box proteins; however, gene duplication was not found among these proteins. Phylogenetic analysis revealed that F-box proteins having similar functional domain was highly diverse form each other showing the possibility of various function. Analysis also found that MPH_00568 and MPH_05531 were closely related to rice blast fungus F-box protein MGG_00768 and MGG_13065, respectively, may play an important role for blast disease development.
This genome-wide analysis of F-box proteins will be useful for characterization of candidate F-box proteins to understand the molecular mechanisms leading to disease development of M. phaseolina in the host plants.
在真菌中，与其他真核生物一样，蛋白质周转是控制各种细胞功能的重要细胞过程。泛素 - 蛋白酶体途径降解一些选定的细胞内蛋白质，F - 盒蛋白是控制蛋白质降解的重要成分之一。F - 盒蛋白在不同的模式植物中已得到充分研究，然而，它们在真菌中的功能尚不清楚。本研究旨在鉴定菜豆壳球孢菌中参与疾病发展的蛋白质降解相关基因。
在本研究中，通过计算机模拟研究来了解F - 盒蛋白在包括菜豆壳球孢菌在内的致病真菌中的分布。全基因组分析表明，菜豆壳球孢菌在其整个染色体中含有31个F - 盒蛋白。此外，分别从禾柄锈菌、玉米炭疽菌、玉米黑粉菌和致病疫霉中鉴定出17、37、16和21个F - 盒蛋白。分析表明，选择性真菌基因组除了F - 盒结构域外还包含几个其他功能域。序列比对显示几种F - 盒蛋白中存在氨基酸替代；然而，在这些蛋白中未发现基因重复。系统发育分析表明，具有相似功能域的F - 盒蛋白彼此高度不同，显示出具有多种功能的可能性。分析还发现，MPH_00568和MPH_05531分别与稻瘟病菌F - 盒蛋白MGG_00768和MGG_13065密切相关，可能在稻瘟病发展中起重要作用。
这种对F - 盒蛋白的全基因组分析将有助于鉴定候选F - 盒蛋白，以了解导致菜豆壳球孢菌在宿主植物中发病的分子机制。
