Chirurgie Maxillo-Faciale, stomatologie et odontologie hospitalière, CHU Besançon, 25000, Besançon, France.
UMR1098, RIGHT Interactions Greffon-Hôte-Tumeur/Ingénierie Cellulaire et Génique, Univ. Bourgogne Franche-Comté, INSERM, EFS BFC, 25000, Besançon, France.
Mol Biol Rep. 2021 Apr;48(4):3799-3812. doi: 10.1007/s11033-021-06299-9. Epub 2021 Mar 24.
Since the discovery of dental pulp stem cells, a lot of teams have expressed an interest in dental pulp regeneration. Many approaches, experimental models and biological explorations have been developed, each including the use of stem cells and scaffolds with the final goal being clinical application in humans. In this review, the authors' objective was to compare the experimental models and strategies used for the development of biomaterials for tissue engineering of dental pulp with stem cells. Electronic queries were conducted on PubMed using the following terms: pulp regeneration, scaffold, stem cells, tissue engineering and biomaterial. The extracted data included the following information: the strategy envisaged, the type of stem cells, the experimental models, the exploration or analysis methods, the cytotoxicity or viability or proliferation cellular tests, the tests of scaffold antibacterial properties and take into account the vascularization of the regenerated dental pulp. From the 71 selected articles, 59% focused on the "cell-transplantation" strategy, 82% used in vitro experimentation, 58% in vivo animal models and only one described an in vivo in situ human clinical study. 87% used dental pulp stem cells. A majority of the studies reported histology (75%) and immunohistochemistry explorations (66%). 73% mentioned the use of cytotoxicity, proliferation or viability tests. 48% took vascularization into account but only 6% studied the antibacterial properties of the scaffolds. This article gives an overview of the methods used to regenerate dental pulp from stem cells and should help researchers create the best development strategies for research in this field.
自牙髓干细胞被发现以来，许多团队对牙髓再生表现出了浓厚的兴趣。许多方法、实验模型和生物学探索已经被开发出来，每种方法都包括使用干细胞和支架，最终目标是在人类中进行临床应用。在这篇综述中，作者的目的是比较用于牙髓组织工程的干细胞生物材料的开发的实验模型和策略。在 PubMed 上使用以下术语进行电子查询：牙髓再生、支架、干细胞、组织工程和生物材料。提取的数据包括以下信息：预期的策略、干细胞的类型、实验模型、探索或分析方法、细胞毒性或活力或增殖细胞测试、支架抗菌性能测试以及考虑再生牙髓的血管化。从 71 篇选定的文章中，59%的文章侧重于“细胞移植”策略，82%的文章在体外进行实验，58%的文章在体内进行动物模型实验，只有一篇描述了体内原位的人类临床研究。87%的文章使用牙髓干细胞。大多数研究报告了组织学（75%）和免疫组织化学探索（66%）。73%提到了使用细胞毒性、增殖或活力测试。48%考虑了血管化，但只有 6%研究了支架的抗菌性能。本文概述了从干细胞中再生牙髓的方法，应有助于研究人员为该领域的研究创造最佳的发展策略。
