Department of Chemistry and Chemical Engineering, Chalmers University of Technology, 41296 Gothenburg, Sweden; email:
Annu Rev Anal Chem (Palo Alto Calif). 2021 Jul 27;14(1):281-297. doi: 10.1146/annurev-anchem-091420-090751.
Here, the research field of nanoplasmonic sensors is placed under scrutiny, with focus on affinity-based detection using refractive index changes. This review describes how nanostructured plasmonic sensors can deliver unique advantages compared to the established surface plasmon resonance technique, where a planar metal surface is used. At the same time, it shows that these features are actually only useful in quite specific situations. Recent trends in the field are also discussed and some devices that claim extraordinary performance are questioned. It is argued that the most important challenges are related to limited receptor affinity and nonspecific binding rather than instrumental performance. Although some nanoplasmonic sensors may be useful in certain situations, it seems likely that conventional surface plasmon resonance will continue to dominate biomolecular interaction analysis. For detection of analytes in complex samples, plasmonics may be an important tool, but probably not in the form of direct refractometric detection.
在这里，我们将仔细研究纳米等离子体传感器的研究领域，重点关注基于折射率变化的亲和力检测。本综述介绍了与传统的表面等离子体共振技术（其中使用平面金属表面）相比，纳米结构等离子体传感器如何提供独特的优势。同时，它表明这些特性实际上仅在特定情况下才有用。还讨论了该领域的最新趋势，并对一些声称具有非凡性能的设备提出了质疑。有人认为，最重要的挑战与受体亲和力有限和非特异性结合有关，而不是与仪器性能有关。尽管在某些情况下，一些纳米等离子体传感器可能有用，但传统的表面等离子体共振似乎很可能继续主导生物分子相互作用分析。对于复杂样品中分析物的检测，等离子体学可能是一个重要的工具，但可能不是直接折射检测的形式。
