Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, P. R. China.
Methods Mol Biol. 2022;2393:15-35. doi: 10.1007/978-1-0716-1803-5_2.
Localized surface plasmon resonance (LSPR) associated with metal nanostructures has developed into highly useful sensor techniques. LSPR spectroscopy often shows absorption peaks which could be used for biomedical detection. Here we report nanoplasmonic sensors using LSPR on nanostructures such as nanoparticles, nanocups, and nanocones to recognize biomolecular. These sensors can be modified for quantitative detection of explosives and evaluation of enzymatic activity. Electrochemical LSPR sensors can also be designed by coupling electrochemistry and LSPR spectroscopy measurements for biochemical detection. Multiple sensing information can be obtained and electrochemical LSPR property can be investigated for biosensors. In some applications, the electrochemical LSPR biosensor can be used to quantify heavy metal ions, neurotransmitters, and sialic acid. The biosensors exhibit better performance than those of conventional optical LSPR measurements. With multitransducers, the nanoplasmonic biosensor can provide a promising approach for biochemical detection in environmental monitoring, healthcare diagnostics, and food quality control.
局部表面等离子体共振（LSPR）与金属纳米结构相关，已发展成为非常有用的传感器技术。LSPR 光谱通常显示吸收峰，可用于生物医学检测。在这里，我们报告了使用纳米粒子、纳米杯和纳米锥等纳米结构上的 LSPR 进行生物分子识别的纳米等离子体传感器。这些传感器可以进行定量检测爆炸物和评估酶活性的修饰。通过将电化学和 LSPR 光谱测量相结合，也可以设计电化学 LSPR 传感器用于生化检测。可以获得多种传感信息，并可以研究用于生物传感器的电化学 LSPR 特性。在某些应用中，电化学 LSPR 生物传感器可用于定量检测重金属离子、神经递质和唾液酸。与传统的光学 LSPR 测量相比，生物传感器具有更好的性能。通过多换能器，纳米等离子体生物传感器为环境监测、医疗诊断和食品质量控制中的生化检测提供了一种很有前途的方法。
