Department of Otolaryngology Head and Neck Surgery, Beiijng Anzhen Hospital Affiliated with Capital Medical University, 2 Anzhen Road, Chaoyang District, Beijing, 100029, China.
Department of Otolaryngology Head and Neck Surgery, Beiijng Anzhen Hospital Affiliated with Capital Medical University, 2 Anzhen Road, Chaoyang District, Beijing, 100029, China.
Int J Pediatr Otorhinolaryngol. 2021 May;144:110669. doi: 10.1016/j.ijporl.2021.110669. Epub 2021 Mar 5.
To investigate the expression and correlation of vascular endothelial growth factor (VEGF) and its receptor with hypoxia-inducible factor-1 α (HIF-1 α) in otitis media with effusion (OME).
A rat model of OME was induced by injection of lipopolysaccharide (LPS) into the middle ear. Hematoxylin and eosin (HE) staining was used to observe the pathomorphological changes of the tympanic cavity in the middle ear of rats. Immunohistochemistry (IHC), western blotting and RT-qPCR were used to determine the mRNA and protein expression of VEGF, VEGFR-1, VEGFR-2 and HIF-1α in mucosa of tympanic cavity mucosa, respectively.
In the OME group, the epithelial space of the middle ear mucosa was significantly thickened and infiltration of a large number of inflammatory cells was found on postoperative day (POD), and the otitis media basically subsided 2 weeks after operation. VEGF mRNA expression was significantly increased on POD 1, and its protein expression peaked on POD 3. HIF-1α mRNA expression was significantly increased and peaked on POD 1, while its protein expression began to increase on POD 3 and was significantly expressed in the middle ear mucosal epithelium. HIF-1α mRNA showed a positive correlation with VEGF mRNA and VEGFR-1 mRNA expression.
VEGF mainly plays a role in the acute phase of OME, and it is abundantly expressed mediated by HIF-1α. And then it play a role in vasodilatation and increase of vascular permeability, thus promoting the generation of middle ear effusion.
研究血管内皮生长因子（VEGF）及其受体与缺氧诱导因子-1α（HIF-1α）在分泌性中耳炎（OME）中的表达及相关性。
采用向中耳内注射脂多糖（LPS）的方法建立大鼠 OME 模型。苏木精-伊红（HE）染色观察大鼠中耳鼓室腔的病理形态学变化。免疫组织化学（IHC）、Western blot 和 RT-qPCR 分别用于检测 VEGF、VEGFR-1、VEGFR-2 和 HIF-1α在鼓膜黏膜中的 mRNA 和蛋白表达。
OME 组大鼠术后第 1 天（POD）中耳黏膜上皮间隙明显增厚，大量炎性细胞浸润，术后第 2 周基本消退。VEGF mRNA 表达在 POD1 时明显增加，其蛋白表达在 POD3 时达到高峰。HIF-1α mRNA 表达在 POD1 时明显增加，达高峰，其蛋白表达在 POD3 时开始增加，并在中耳黏膜上皮中表达明显。HIF-1α mRNA 与 VEGF mRNA 和 VEGFR-1 mRNA 表达呈正相关。
VEGF 主要在 OME 的急性期发挥作用，由 HIF-1α介导大量表达。继而发挥血管扩张和增加血管通透性的作用，从而促进中耳积液的产生。
