Department of Otolaryngology Head and Neck Surgery, Beijing Anzhen Hospital, Capital Medical University, No. 2 Anzhen Road, Beijing, 100029, People's Republic of China.
Eur Arch Otorhinolaryngol. 2019 Jul;276(7):1889-1895. doi: 10.1007/s00405-019-05400-0. Epub 2019 Mar 27.
Some studies have demonstrated that vascular endothelial growth factor (VEGF) plays a critical role in the pathogenesis of otitis media with effusion (OME) in animal models. However, the levels of VEGF and its receptors in adult OME have not been clarified. Our study was designed to detect the levels of VEGF and its receptors in adult OME and explore their relationship with effusion types, duration and prognosis of OME.
61 patients with secretory otitis media were enrolled including 21 males and 40 females, with an average age of 54.7 ± 17.5 years. The middle-ear effusions were collected by tympanocentesis or myringotomy. The protein concentrations were determined by enzyme-linked immunosorbent assay and messenger RNA by real-time quantitative PCR.
VEGF level was higher in AOME group, but not correlated with the recurrence of OME. VEGFR1 and VEGFR2 levels were lower in recurrent group compared with non-recurrent group. VEGFR2 level was higher in serous effusions than mucoid effusions. VEGF messenger RNA was positively correlated both with HIF-1α and MUC5B.
VEGF and its receptors function to induce the production of middle-ear effusions (MEEs) at acute stage of OME rather than chronic or recurrent stage, which is mainly mediated by HIF-1α pathway. The formation of mucoid effusions is associated with MUC5B and VEGFR2, but not with duration and recurrence of OME.
一些研究表明血管内皮生长因子（VEGF）在动物模型中耳积液（OME）的发病机制中起关键作用。然而，成人OME 中 VEGF 及其受体的水平尚未阐明。本研究旨在检测成人 OME 中 VEGF 及其受体的水平，并探讨其与渗出液类型、OME 持续时间和预后的关系。
纳入 61 例分泌性中耳炎患者，其中男性 21 例，女性 40 例，平均年龄 54.7±17.5 岁。经鼓膜穿刺或鼓膜切开术收集中耳积液。酶联免疫吸附试验测定蛋白浓度，实时定量 PCR 测定信使 RNA。
急性OME 组 VEGF 水平较高，但与 OME 复发无关。与非复发组相比，复发组 VEGFR1 和 VEGFR2 水平较低。浆液性渗出液中 VEGFR2 水平高于黏液性渗出液。VEGF 信使 RNA 与 HIF-1α和 MUC5B 呈正相关。
VEGF 及其受体在 OME 的急性阶段发挥作用，诱导中耳渗出液（MEE）的产生，而不是慢性或复发性阶段，主要通过 HIF-1α途径介导。黏液性渗出液的形成与 MUC5B 和 VEGFR2 有关，而与 OME 的持续时间和复发无关。
