Daegu Gyeongbuk Medical Innovation Foundation, Laboratory Animal Center, Daegu, Republic of Korea.
Department of Laboratory Medicine, Kyungpook National University, School of Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea.
Biomed Mater. 2021 Apr 9;16(4). doi: 10.1088/1748-605X/abf1a8.
There is a need for effective wound healing through rapid wound closure, reduction of scar formation, and acceleration of angiogenesis. Hydrogel is widely used in tissue engineering, but it is not an ideal solution because of its low vascularization capability and poor mechanical properties. In this study, gelatin methacrylate (GelMA) was tested as a viable option with tunable physical properties. GelMA hydrogel incorporating a vascular endothelial growth factor (VEGF) mimicking peptide was successfully printed using a three-dimensional (3D) bio-printer owing to the shear-thinning properties of hydrogel inks. The 3D structure of the hydrogel patch had high porosity and water absorption properties. Furthermore, the bioactive characterization was confirmed by cell culture with mouse fibroblasts cell lines (NIH 3T3) and human umbilical vein endothelial cells. VEGF peptide, which is slowly released from hydrogel patches, can promote cell viability, proliferation, and tubular structure formation. In addition, a pig skin wound model was used to evaluate the wound-healing efficacy of GelMA-VEGF hydrogel patches; the results suggest that the GelMA-VEGF hydrogel patch can be used for wound dressing.
需要通过快速伤口闭合、减少疤痕形成和加速血管生成来实现有效的伤口愈合。水凝胶广泛应用于组织工程中，但由于其血管生成能力低和机械性能差，并不是理想的解决方案。在这项研究中，明胶甲基丙烯酰胺（GelMA）因其具有可调的物理特性而被测试为一种可行的选择。由于水凝胶油墨的剪切稀化特性，血管内皮生长因子（VEGF）模拟肽的 GelMA 水凝胶成功地使用 3D 生物打印机进行了打印。水凝胶贴剂的 3D 结构具有高孔隙率和吸水性。此外，通过与小鼠成纤维细胞系（NIH 3T3）和人脐静脉内皮细胞的细胞培养证实了生物活性特征。从水凝胶贴片中缓慢释放的 VEGF 肽可以促进细胞活力、增殖和管状结构的形成。此外，还使用猪皮伤口模型评估了 GelMA-VEGF 水凝胶贴剂的伤口愈合功效；结果表明，GelMA-VEGF 水凝胶贴剂可用于伤口敷料。
