Department of Orthopedics, Daping Hospital, Army Medical University, Chongqing, PR China.
Medicine (Baltimore). 2021 Mar 26;100(12):e24765. doi: 10.1097/MD.0000000000024765.
MicroRNA (miR)-26a-5p is an oncogene significantly associated with osteosarcoma. We try to evaluate expression of circulating miR-26a-5p in osteosarcoma patients and evaluate its significance.A total of 243 consecutive osteosarcoma patients and 96 healthy participates were enrolled. Circulating miR-26a-5p levels were evaluated by using real-time quantitative reverse transcription polymerase chain reactions (RT-PCR). The association between circulating miR-26a-5p level and survival outcomes was evaluated by univariate and multivariate analysis.Circulating miR-26a-5p levels in osteosarcoma patients was significantly higher than that of healthy volunteers (P < .05). Upregulated miR-26a-5p was significantly related to advanced cancer and metastasis (both P < .05). Moreover, patients with a high serum miR-26a-5p had a poorer overall survival than those with a low serum miR-26a-5p levels (P < .05). Circulating miR-26a-5p level also been showed as independent risk factor for osteosarcoma in multivariate analysis (hazard ratio [HR], 0.38; 95% confidence interval [CI]: 0.11-0.98; P < .01).Circulating miR-26a-5p was significantly upregulated in osteosarcoma patients and remarkably associated with poor prognosis, indicating that circulating miR-26a-5p might serve as a useful diagnostic and prognostic biomarker for osteosarcoma.
微小 RNA（miR）-26a-5p 是一种与骨肉瘤显著相关的癌基因。我们试图评估骨肉瘤患者循环 miR-26a-5p 的表达，并评估其意义。共纳入 243 例连续骨肉瘤患者和 96 例健康志愿者。采用实时定量逆转录聚合酶链反应（RT-PCR）评估循环 miR-26a-5p 水平。通过单因素和多因素分析评估循环 miR-26a-5p 水平与生存结局的关系。骨肉瘤患者循环 miR-26a-5p 水平明显高于健康志愿者（P<0.05）。上调的 miR-26a-5p 与晚期癌症和转移明显相关（均 P<0.05）。此外，血清 miR-26a-5p 水平高的患者总生存率低于血清 miR-26a-5p 水平低的患者（P<0.05）。多因素分析显示，循环 miR-26a-5p 水平也是骨肉瘤的独立危险因素（危险比[HR]，0.38；95%置信区间[CI]：0.11-0.98；P<0.01）。循环 miR-26a-5p 在骨肉瘤患者中明显上调，与不良预后显著相关，表明循环 miR-26a-5p 可能作为骨肉瘤有用的诊断和预后生物标志物。
