Mao Ling, Wu Xiaoweng, Gong Zhengpeng, Yu Ming, Huang Zhi
The Laboratory of Head and Neck Cancer Research, Hospital and School of Stomatology, Guizhou Medical University, Guiyang, 550004, People's Republic of China.
Imaging department, Liaocheng People's Hospital, Liaocheng, China.
World J Surg Oncol. 2021 Mar 24;19(1):88. doi: 10.1186/s12957-021-02190-w.
BACKGROUND/OBJECTIVE: Accumulated evidence has demonstrated that aerobic glycolysis serves as a regulator of tumor cell growth, invasion, and angiogenesis. Herein, we explored the role of protein disulfide isomerase family 6 (PDIA6) in the aerobic glycolysis and the progression of oral squamous cell carcinoma (OSCC).
The expression pattern of PDIA6 in OSCC tissues was determined by qPCR and western blotting. Lentivirus and small interfering RNAs (siRNAs) were introduced into cells to upregulate and downregulate PDIA6 expression. CCK-8, flow cytometry, transwell, and xenotransplantation models were applied to detect cell proliferation, apoptosis, migration, invasion, and tumorigenesis, respectively.
A high expression pattern of PDIA6 was observed in OSCC tissues, which was closely associated with lower overall survival and malignant clinical features in OSCC. Compared with the control group, overexpression of PDIA6 induced significant enhancements in cell growth, migration, invasiveness, and tumorigenesis and decreased cell apoptosis, while knockdown of PDIA6 caused opposite results. In addition, overexpression of PDIA6 increased glucose consumption, lactate production, and ATP level in OSCC cells.
This study demonstrated that PDIA6 expression was elevated in OSCC tissues, and overexpression of it promoted aerobic glycolysis and OSCC progression.
背景/目的：越来越多的证据表明，有氧糖酵解是肿瘤细胞生长、侵袭和血管生成的调节因子。在此，我们探讨了蛋白二硫键异构酶家族6（PDIA6）在有氧糖酵解及口腔鳞状细胞癌（OSCC）进展中的作用。
通过qPCR和蛋白质印迹法检测PDIA6在OSCC组织中的表达模式。将慢病毒和小干扰RNA（siRNAs）导入细胞以上调和下调PDIA6的表达。分别应用CCK-8、流式细胞术、Transwell和异种移植模型检测细胞增殖、凋亡、迁移、侵袭和肿瘤发生情况。
在OSCC组织中观察到PDIA6的高表达模式，这与OSCC患者较低的总生存率和恶性临床特征密切相关。与对照组相比，PDIA6过表达显著促进细胞生长、迁移、侵袭和肿瘤发生，并减少细胞凋亡，而敲低PDIA6则产生相反的结果。此外，PDIA6过表达增加了OSCC细胞的葡萄糖消耗、乳酸生成和ATP水平。
本研究表明，OSCC组织中PDIA6表达升高，其过表达促进有氧糖酵解和OSCC进展。
