Chen Xiudong, Liu Qing, Chen Juan, Liu Yuhai
Department of Pediatric, Zaozhuang Maternal and Child Health Care Hospital, Zaozhuang City, Shandong Province, 277100, People's Republic of China.
Int J Gen Med. 2021 Mar 17;14:895-902. doi: 10.2147/IJGM.S291239. eCollection 2021.
Pneumonia is a common infection of the lung parenchyma in children, and early and accurate diagnosis of childhood pneumonia (CP) is important for implementing appropriate preventive and treatment strategies. This study aimed to evaluate the diagnostic value of the combination of long non-coding RNA (lncRNA) RP11-248E9.5, RP11-456D7.1, c-reactive protein (CRP), neutrophil-to-lymphocyte ratio (NLR), and platelet-to-lymphocyte ratio (PLR) in CP.
A total of 50 healthy children (HC) and 100 CP patients were enrolled. The serum expression of RP11-248e9.5 and RP11-456d7.1 was detected by qRT-PCR. The white blood cell (WBC), hemoglobin (HB), platelet (PLT), neutrophil, and lymphocyte were analyzed by automated hematology analyzer. The serum levels of CRP and procalcitonin (PCT) were analyzed by automatic biochemical analyzer. The receiver operating characteristic (ROC) curves were applied to evaluate the diagnostic value in CP.
The NLR and PLR, expression of RP11-248E9.5 and RP11-456D7.1, and serum levels of CRP and PCT were significantly higher in the CP group than those in the HC group. Both RP11-248E9.5 (AUC, 0.86; sensitivity, 84%; specificity, 78%) and RP11-456D7.1 (AUC, 0.89; sensitivity, 79%; specificity, 92%) exhibited certain diagnostic value in CP. The diagnostic values of PCT, CRP, NLR and PLR in CP were limited by low sensitivity (≤ 71%). The combination of multiple indicators improved the diagnostic value. The combination of RP11-248E9.5, RP11-456D7.1, CRP, NLR, and PLR had the best diagnostic value in CP (AUC, 0.992; Sensitivity, 0.97; Specificity, 0.99).
The combination of RP11-248E9.5, RP11-456D7.1, CRP, NLR, and PLR was a potential diagnostic strategy for CP.
肺炎是儿童肺实质常见的感染性疾病，早期准确诊断儿童肺炎（CP）对于实施恰当的预防和治疗策略至关重要。本研究旨在评估长链非编码RNA（lncRNA）RP11 - 248E9.5、RP11 - 456D7.1、C反应蛋白（CRP）、中性粒细胞与淋巴细胞比值（NLR）以及血小板与淋巴细胞比值（PLR）联合检测在儿童肺炎中的诊断价值。
共纳入50例健康儿童（HC）和100例儿童肺炎患者。采用qRT - PCR检测血清中RP11 - 248e9.5和RP11 - 456d7.1的表达。通过自动血液分析仪分析白细胞（WBC）、血红蛋白（HB）、血小板（PLT）、中性粒细胞和淋巴细胞。采用自动生化分析仪分析血清中CRP和降钙素原（PCT）的水平。应用受试者工作特征（ROC）曲线评估其在儿童肺炎中的诊断价值。
儿童肺炎组的NLR、PLR、RP11 - 248E9.5和RP11 - 456D7.1的表达以及血清CRP和PCT水平均显著高于健康儿童组。RP11 - 248E9.5（曲线下面积[AUC]，0.86；灵敏度，84%；特异度，78%）和RP11 - 456D7.1（AUC，0.89；灵敏度，79%；特异度，92%）在儿童肺炎中均表现出一定的诊断价值。PCT、CRP、NLR和PLR在儿童肺炎中的诊断价值因灵敏度较低（≤71%）而受限。多项指标联合可提高诊断价值。RP11 - 248E9.5、RP11 - 456D7.1、CRP、NLR和PLR联合检测在儿童肺炎中具有最佳诊断价值（AUC，0.992；灵敏度，0.97；特异度，0.99）。
RP11 - 248E9.5、RP11 - 456D7.1、CRP、NLR和PLR联合检测是儿童肺炎潜在的诊断策略。
