The First School of Clinical Medicine, Lanzhou University, Lanzhou, 730000, China.
Department of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou, 730000, China.
Ir J Med Sci. 2022 Apr;191(2):509-517. doi: 10.1007/s11845-021-02604-4. Epub 2021 Mar 24.
Nucleic acid testing is a reliable method for diagnosing viral infection in clinical samples. However, when the number of cases is huge and there are individual differences in the virus itself, the probability of false-negative results increases. With the advancement in research on the new coronavirus, new detection technologies that use serum-specific antibodies as detection targets have been developed. These detection technologies have high efficiency and shorter turnaround time, which ultimately shortens the time required for diagnosis. This article summarizes the methods that have been reported to date for the detection of the new coronavirus and discusses their principles and technical characteristics.
Compare the advantages and disadvantages of various SARS-CoV-2 detection methods and analyze their principles.
Searched reports on SARS-CoV-2 detection methods published so far, extracted the data and analyzed them. Use the primer blast function of NCBI to analyze the primers used in qRT-PCR detection.
The detection sensitivity was the highest when nucleocapsid protein gene was used as the target, reaching 96.6%. The detection efficiency of the remaining targets ranged from 66.7% to 96.0%. Various new detection methods, like Serum specific antibody detection, can speed up the test time. However, due to the complexity of the method and higher testing requirements, it seems that it cannot be used as a complete replacement for qRT-PRC testing.
With the advancement of technology and the improvement of methods, the detection methods of SARSCoV-2 have become more mature. These advances provided great help to the detection of SARS-CoV-2.
核酸检测是临床样本中诊断病毒感染的可靠方法。然而，当病例数量巨大且病毒本身存在个体差异时，假阴性结果的概率会增加。随着对新型冠状病毒研究的进展，已经开发出了使用血清特异性抗体作为检测靶点的新型检测技术。这些检测技术具有高效率和更短的周转时间，最终缩短了诊断所需的时间。本文总结了迄今为止报道的用于检测新型冠状病毒的方法，并讨论了它们的原理和技术特点。
比较各种 SARS-CoV-2 检测方法的优缺点，并分析其原理。
检索了迄今为止发表的有关 SARS-CoV-2 检测方法的报告，提取数据并进行分析。使用 NCBI 的引物 Blast 功能分析 qRT-PCR 检测中使用的引物。
以核衣壳蛋白基因为检测靶点的检测灵敏度最高，达到 96.6%。其余靶标的检测效率范围为 66.7%至 96.0%。各种新的检测方法，如血清特异性抗体检测，可以加快测试时间。然而，由于方法的复杂性和更高的测试要求，它似乎不能完全替代 qRT-PCR 检测。
随着技术的进步和方法的改进，SARS-CoV-2 的检测方法变得更加成熟。这些进展为 SARS-CoV-2 的检测提供了很大的帮助。
