CICECO - Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, Aveiro, 3810-193, Portugal.
Adv Healthc Mater. 2021 May;10(10):e2100266. doi: 10.1002/adhm.202100266. Epub 2021 Mar 24.
The therapeutic effectiveness and biological relevance of technologies based on adherent cells depend on platforms that enable long-term culture in controlled environments. Liquid-core capsules have been suggested as semipermeable moieties with spatial homogeneity due to the high mobility of all components in their core. The lack of cell-adhesive sites in liquid-core structures often hampers their use as platforms for stem cell-based technologies for long-term survival and cell-directed self-organization. Here, the one-step fast formation of robust polymeric capsules formed by interfacial complexation of oppositely charged polyelectrolytes in an all-aqueous environment, compatible with the simultaneous encapsulation of mesenchymal stem/stromal cells (MSCs) and microcarriers, is described. The adhesion of umbilical cord MSCs to polymeric microcarriers enables their aggregation and culture for more than 21 days in capsules prepared either manually by dropwise addition, or by scalable electrohydrodynamic atomization, generating robust and stable capsules. Cell aggregation and secretion overtime can be tailored by providing cells with static or dynamic (bioreactor) environments.
基于贴壁细胞的技术的治疗效果和生物学相关性取决于能够在受控环境中进行长期培养的平台。由于其核心中所有成分的高迁移率，液体芯胶囊已被提议作为具有空间均一性的半透性部分。由于液体芯结构中缺乏细胞附着位点，因此它们通常不能用作基于干细胞的技术的平台，以实现长期存活和细胞定向的自组织。在这里，描述了在全水相环境中通过带相反电荷的聚电解质界面络合一步快速形成稳定的聚合物胶囊的方法，该方法同时适用于间充质干细胞/基质细胞（MSCs）和微载体的包封。脐带 MSC 与聚合物微载体的黏附能够使它们在通过逐滴添加或通过可扩展的电动力学雾化手工制备的胶囊中聚集和培养超过 21 天，从而生成稳定的胶囊。通过为细胞提供静态或动态（生物反应器）环境，可以调整细胞的聚集和分泌随时间的变化。
