Department of Horticultural Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, 9177948978, Iran.
Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, 9177948978, Iran.
Chem Biodivers. 2021 Apr;18(4):e2001044. doi: 10.1002/cbdv.202001044. Epub 2021 Mar 25.
Mentha longifolia L. is well-known to be one of the most pervasive wild-growing species of the Lamiaceae family, which has extensive beneficial properties in the fields of pharmacology and biological products. In the present study, the correlation between Inter-simple sequence repeat (ISSR) markers and morpho-chemical parameters of twenty different M. longifolia accessions (MLACs) were assessed. The geographic information system (GIS) has been employed to interpret the original habitat of the accessions in Iran. ISSR analysis indicated a remarkable difference in the studied accessions, segregated them into three main groups, constructed by an unweighted pair-group method with arithmetic (UPGMA) and principal coordinate analysis (PCoA). A total of 89 bands were generated by 12 ISSR primers, among which 82 (91.97 %) of them were polymorphic. The cluster analysis based on agro-morphological data scattered MLACs into two main groups. The essential oils (EOs) were analyzed through GC/FID/MS, and four chemotypes were characterized according to the major constituents. Pulegone ranged from 0.17 to 69.50 % was the main oil constituent with the highest content. Also, HPLC-PDA was employed to identify and to quantify the phenolic compounds in the MeOH extracts of MLACs. Heatmap cluster based on phenolic compounds produced three main categories of accessions. The components identified in the extracts were rosmarinic acid, rutin, vanillic acid, ferulic acid, chlorogenic acid, caffeic acid, 3,4-dihydroxybenzoic acid, 2,5-dihydroxybenzoic acid, and p-coumaric acid, which among them rosmarinic acid (RA) varied from 39.16 to 261.55 mg/100 g (DW) as a predominant constituent. Subsequently, multiple regression analyses between ISSR fragments and morpho-chemical data illustrated considerable relationships in the plant materials. The high variation and correlation observed in metabolic and phenotypic traits of MLACs establish an adequate source to conduct reserves conservation programs.
长叶薄荷（Mentha longifolia L.）是唇形科中分布最广的野生物种之一，在药理学和生物制品领域具有广泛的有益特性。本研究评估了 20 种不同长叶薄荷（MLAC）的 ISSR 标记与形态化学参数之间的相关性。地理信息系统（GIS）被用于解释伊朗引种的原始生境。ISSR 分析表明，研究中的引种存在显著差异，将它们分为三个主要组群，通过算术加权对组平均法（UPGMA）和主坐标分析（PCoA）构建。用 12 个 ISSR 引物共产生 89 个条带，其中 82 个（91.97%）为多态性条带。基于农艺形态学数据的聚类分析将 MLAC 分为两个主要组群。通过 GC/FID/MS 分析精油（EO），并根据主要成分确定了 4 种化学型。胡椒酮（pulegone）的含量范围为 0.17 至 69.50%，是含量最高的主要油分。此外，还采用 HPLC-PDA 法鉴定和定量 MLAC 甲醇提取物中的酚类化合物。基于酚类化合物的热图聚类产生了三个主要的引种组群。提取物中鉴定出的成分有迷迭香酸、芦丁、香草酸、阿魏酸、绿原酸、咖啡酸、3,4-二羟基苯甲酸、2,5-二羟基苯甲酸和对香豆酸，其中迷迭香酸（RA）的含量为 39.16 至 261.55 mg/100 g（DW），是主要成分。随后，ISSR 片段与形态化学数据之间的多元回归分析表明，植物材料之间存在相当大的关系。MLACs 的代谢和表型性状的高度变异性和相关性为开展资源保护计划提供了充足的来源。
